Human Erythropoietin R PE-conjugated Antibody

Catalog #: FAB3072P Datasheet / COA / SDS

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FAB3072P

Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry.

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Human Erythropoietin R PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Epo R in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) Epo, rhTpo R, or recombinant mouse Epo R.

Source

Monoclonal Mouse IgG₁ Clone # 38421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Ala25-Pro250
Accession # P19235

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

TF‑1 human erythroleukemic cell line; KU812 human Chronic Myelogenous Leukemic cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R PE-conjugated Monoclonal Antibody (Catalog # FAB3072P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Erythropoietin R in KU812 cells by Flow Cytometry KU812 cells were stained with Mouse Anti-Human Erythropoietin R PE‑conjugated Monoclonal Antibody (Catalog # FAB3072P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name

Erythropoietin Receptor

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Alternate Names

EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358