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Human ETV5 Antibody

Catalog #: MAB7107
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7107
MAB7107-SP
ETV5 in BT‑20 Human Cell Line.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ETV5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ETV5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ETV1 or rhETV2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 654404
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ETV5
Asn119-Ser223
Accession # P41161
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ETV5 antibody in BT-20 Human Cell Line by Immunocytochemistry (ICC). View Larger

ETV5 in BT‑20 Human Cell Line. ETV5 was detected in immersion fixed BT-20 human breast cancer cell line using Mouse Anti-Human ETV5 Monoclonal Antibody (Catalog # MAB7107) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ETV5

ETS translocation variant 5 (ETV5), also known as ERM, is an approximately 70 kDa member of the ETS transcription factor family. The expression of ETV5 in Sertoli, granulosa, and cumulus cells is important for the ability of these cells to support spermatogonia stem cell and oocyte development. ETV5 is upregulated at the invasive front of various carcinomas, and it plays a role in tumor cell invasiveness. It is a target of chromosomal translocation in prostate cancer and forms a fusion protein with TMPRSS2. It is also required for branching morphogenesis during early kidney development. ETV5 contains a single Ets DNA-binding domain (aa 367-452). Within aa 119-223, human ETV5 shares 92% aa sequence identity with mouse and rat ETV5.

Long Name
ETS Translocation Variant 5
Entrez Gene IDs
2119 (Human); 104156 (Mouse); 303828 (Rat)
Alternate Names
ERM; ERMEts-related protein ERM; ETS translocation variant 5; ets variant 5; ets variant gene 5 (ets-related molecule); ets-related molecule; ETV5

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Citation for Human ETV5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dynamics of chromatin accessibility during TGF-?-induced EMT of Ras-transformed mammary gland epithelial cells
    Authors: M Arase, Y Tamura, N Kawasaki, K Isogaya, R Nakaki, A Mizutani, S Tsutsumi, H Aburatani, K Miyazono, D Koinuma
    Sci Rep, 2017-04-26;7(1):1166.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ChIP

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