Human EVI-1 Antibody

Catalog #: MAB7506 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7506
MAB7506-SP

Detection of Human EVI‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EVI-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EVI-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 702319

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human EVI-1
Gly241-Met430
Accession # Q03112

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human EVI‑1 by Western Blot. Western blot shows lysates of MDA-MB-231 human breast cancer cell line and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EVI-1 Monoclonal Antibody (Catalog # MAB7506) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EVI-1 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EVI-1

Ectopic virus integration site 1 (EVI1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI1 shares 94% aa sequence identity with mouse and rat EVI1.

Long Name

Ecotropic Viral Integration Site 1

Entrez Gene IDs

2122 (Human); 14013 (Mouse); 294924 (Rat)

Alternate Names

AML1-EVI-1 fusion protein; AML1-EVI-1; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI1; EVI-1; EVI1MDS1-EVI1; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1 and EVI1 complex locus; MDS1; MECOM; MGC163392; MGC97004; Myelodysplasia syndrome 1 protein; myelodysplasia syndrome 1; Myelodysplasia syndrome-associated protein 1; oncogene EVI1; PRDM3; zinc finger protein Evi1