Home / FABP2/I-FABP / Human FABP2/I-FABP DuoSet ELISA

Human FABP2/I-FABP DuoSet ELISA

Catalog #: DY3078
28 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY3078
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human FABP2 / I-FABP ELISA Standard Curve
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Product Details
Procedure
Citations (28)
FAQs
Supplemental Products
Reviews (3)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human FABP2/I-FABP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FABP2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

 

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

 

Scientific Data

N/A Human FABP2 / I-FABP ELISA Standard Curve View Larger

Human FABP2 / I-FABP ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name:
Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs:
2169 (Human); 25598 (Rat)
Alternate Names:
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human FABP2/I-FABP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Toll-like Receptor 2 Mediated Immune Regulation in Simian Immunodeficiency Virus-Infected Rhesus Macaques
    Authors: Boby, N;Williams, KM;Das, A;Pahar, B;
    Vaccines
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  2. Impaired gut microbiota-mediated short-chain fatty acid production precedes morbidity and mortality in people with HIV
    Authors: Sereti, I;Verburgh, ML;Gifford, J;Lo, A;Boyd, A;Verheij, E;Verhoeven, A;Wit, FWNM;Schim van der Loeff, MF;Giera, M;Kootstra, NA;Reiss, P;Vujkovic-Cvijin, I;
    Cell reports
    Species: Human
    Sample Types: Plasma
  3. Circulating Concentrations of Cathelicidin Anti-Microbial Peptide (CAMP) Are Increased during Oral Glucose Tolerance Test
    Authors: Höpfinger, A;Karrasch, T;Schäffler, A;Schmid, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  4. Circulating Levels of Cathelicidin Antimicrobial Peptide (CAMP) Are Affected by Oral Lipid Ingestion
    Authors: Höpfinger, A;Karrasch, T;Schäffler, A;Schmid, A;
    Nutrients
    Species: Human
    Sample Types: Serum
  5. Serum TGF-?1 and CD14 Predicts Response to Anti-TNF-? Therapy in IBD
    Authors: Coufal, S;Kverka, M;Kreisinger, J;Thon, T;Rob, F;Kolar, M;Reiss, Z;Schierova, D;Kostovcikova, K;Roubalova, R;Bajer, L;Jackova, Z;Mihula, M;Drastich, P;Tresnak Hercogova, J;Novakova, M;Vasatko, M;Lukas, M;Tlaskalova-Hogenova, H;Jiraskova Zakostelska, Z;
    Journal of immunology research
    Species: Human
    Sample Types: Serum
  6. Increased intestinal-fatty acid binding protein in obesity-associated type 2 diabetes mellitus
    Authors: DL Tahapary, AI Fatya, F Kurniawan, C Marcella, I Rinaldi, TJE Tarigan, DS Harbuwono, E Yunir, P Soewondo, D Purnamasar
    PLoS ONE, 2023-01-26;18(1):e0279915.
    Species: Human
    Sample Types: Serum
  7. Intestinal Barrier Dysfunction and Microbial Translocation in Patients with First-Diagnosed Atrial Fibrillation
    Authors: L Blöbaum, M Witkowski, M Wegner, S Lammel, PA Schencke, K Jakobs, M Puccini, D Rei beta ner, D Steffens, U Landmesser, U Rauch, J Friebel
    Biomedicines, 2023-01-10;11(1):.
    Species: Human
    Sample Types: Plasma
  8. The role of environmental enteric dysfunction in the pathogenesis of Schistosoma mansoni-associated morbidity in school-aged children
    Authors: J Araújo Fiu, S Colt, L Gambogi de, L Ferreira M, A Gazzinelli, JF Friedman, R Corrêa-Oli
    PloS Neglected Tropical Diseases, 2022-10-05;16(10):e0010837.
    Species: Human
    Sample Types: Plasma
  9. Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria
    Authors: ML Sarangam, R Namazzi, D Datta, C Bond, CPB Vanderpool, RO Opoka, CC John, AL Conroy
    MBio, 2022-09-07;0(0):e0132522.
    Species: Human
    Sample Types: Serum
  10. Intestinal permeability before and after albendazole treatment in low and high socioeconomic status schoolchildren in Makassar, Indonesia
    Authors: AI Amaruddin, JPR Koopman, M Muhammad, K Lenaerts, HMH van Eijk, EAT Brienen, AR Geelen, L van Liesho, S Wahyuni, EJ Kuijper, RD Zwittink, F Hamid, E Sartono, M Yazdanbakh
    Scientific Reports, 2022-03-01;12(1):3394.
    Species: Human
    Sample Types: Serum
  11. Irreversible depletion of intestinal CD4+ T-cells is associated with T-cell activation during chronic HIV infection
    Authors: OE Asowata, A Singh, A Ngoepe, N Herbert, R Fardoos, K Reddy, Y Zungu, F Nene, N Mthabela, D Ramjit, F Karim, K Govender, T Ndung'u, JZ Porterfiel, JH Adamson, FG Madela, VT Manzini, F Anderson, A Leslie, HN Kløverpris
    JCI Insight, 2021-11-22;0(0):.
    Species: Human
    Sample Types: Plasma
  12. Biomarkers of disease severity in patients with visceral leishmaniasis co-infected with HIV
    Authors: GR Ferreira, JR Santos-Oli, ML Silva-Frei, M Honda, DL Costa, AM Da-Cruz, CHN Costa
    Cytokine, 2021-10-27;149(0):155747.
    Species: Human
    Sample Types: Serum
  13. Accuracy of citrulline, I-FABP and D-lactate in the diagnosis of acute mesenteric ischemia
    Authors: A Nuzzo, K Guedj, S Curac, C Hercend, C Bendavid, N Gault, A Tran-Dinh, M Ronot, A Nicoletti, Y Bouhnik, Y Castier, O Corcos, K Peoc'h, SURVI (Str
    Scientific Reports, 2021-09-23;11(1):18929.
    Species: Human
    Sample Types: Plasma
  14. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
    Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
    Antioxidants (Basel), 2021-05-28;10(6):.
    Species: Human
    Sample Types: Urine
  15. Changes in liver steatosis in HIV-positive women are associated with the BMI, but not with biomarkers
    Authors: R Fernandez-, MW Plankey, D Ware, J Bordon
    Cytokine, 2021-05-12;0(0):155573.
    Species: Human
    Sample Types: Plasma
  16. Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection
    Authors: S Novelli, C Lécuroux, C Goujard, J Reynes, A Villemant, L Blum, A Essat, V Avettand-F, O Launay, JM Molina, C Bourgeois, L Meyer
    EBioMedicine, 2020-11-26;62(0):103129.
    Species: Human
    Sample Types: Plasma
  17. Randomised Controlled Trial: Partial Hydrolysation of Casein Protein in Milk Decreases Gastrointestinal Symptoms in Subjects with Functional Gastrointestinal Disorders
    Authors: R Laatikaine, H Salmenkari, T Sibakov, H Vapaatalo, A Turpeinen
    Nutrients, 2020-07-18;12(7):.
    Species: Human
    Sample Types: Plasma
  18. HIV-associated gut dysbiosis is independent of sexual practice and correlates with noncommunicable diseases
    Authors: I Vujkovic-C, O Sortino, E Verheij, J Sklar, FW Wit, NA Kootstra, B Sellers, JM Brenchley, J Ananworani, MSV Loeff, Y Belkaid, P Reiss, I Sereti
    Nat Commun, 2020-05-15;11(1):2448.
    Species: Human
    Sample Types: Plasma
  19. Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations
    Authors: C Lalande, JP Drouin-Cha, AJ Tremblay, P Couture, A Veilleux
    Diabetol Metab Syndr, 2020-04-09;12(0):31.
    Species: Human
    Sample Types: Plasma
  20. Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study
    Authors: S Ma, T Tominaga, K Kanda, K Sugama, C Omae, S Hashimoto, K Aoyama, Y Yoshikai, K Suzuki
    Biomedicines, 2020-03-04;8(3):.
    Species: Human
    Sample Types: Urine
  21. Protective HLA alleles are associated with reduced LPS levels in acute HIV infection with implications for immune activation and pathogenesis
    Authors: DT Claiborne, EP Scully, CD Palmer, JL Prince, GN Macharia, J Kopycinski, CM Michelo, HW Wiener, R Parker, K Nganou-Mak, D Douek, M Altfeld, J Gilmour, MA Price, J Tang, W Kilembe, SA Allen, E Hunter
    PLoS Pathog., 2019-08-26;15(8):e1007981.
    Species: Human
    Sample Types: Plasma
  22. Serum Albumin Is Associated With Higher Inflammation and Carotid Atherosclerosis in Treated Human Immunodeficiency Virus Infection
    Authors: S Dirajlal-F, M Kulkarni, E Bowman, L Shan, A Sattar, N Funderburg, GA McComsey
    Open Forum Infect Dis, 2018-11-17;5(11):ofy291.
    Species: Human
    Sample Types: Serum
  23. Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection
    Authors: Y Guillén, M Noguera-Ju, J Rivera, M Casadellà, AS Zevin, M Rocafort, M Parera, C Rodríguez, M Arumí, J Carrillo, B Mothe, C Estany, J Coll, I Bravo, C Herrero, J Saz, G Sirera, A Torrella, J Navarro, M Crespo, E Negredo, C Brander, J Blanco, ML Calle, NR Klatt, B Clotet, R Paredes
    Mucosal Immunol, 2018-08-31;0(0):.
    Species: Human
    Sample Types: Plasma
  24. High Cellular Monocyte Activation in People Living With Human Immunodeficiency Virus on Combination Antiretroviral Therapy and Lifestyle-Matched Controls Is Associated With Greater Inflammation in Cerebrospinal Fluid
    Authors: T Booiman, FW Wit, I Maurer, D De Frances, CA Sabin, AM Harskamp, M Prins, P Garagnani, C Pirazzini, C Franceschi, D Fuchs, M Gisslén, A Winston, P Reiss, NA Kootstra
    Open Forum Infect Dis, 2017-05-25;4(3):ofx108.
    Species: Human
    Sample Types: Plasma
  25. Intestinal cell damage and systemic immune activation in individuals reporting sensitivity to wheat in the absence of coeliac disease.
    Authors: Uhde M, Ajamian M, Caio G, De Giorgio R, Indart A, Green P, Verna E, Volta U, Alaedini A
    Gut, 2016-07-25;65(12):1930-1937.
    Species: Human
    Sample Types: Serum
  26. Bacteroides are associated with GALT iNKT cell function and reduction of microbial translocation in HIV-1 infection
    Authors: D Paquin-Pro, C Ching, I Vujkovic-C, D Fadrosh, L Loh, Y Huang, M Somsouk, SV Lynch, PW Hunt, DF Nixon, D SenGupta
    Mucosal Immunol, 2016-04-06;0(0):.
    Species: Human
    Sample Types: Plasma
  27. Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression.
    Authors: Claiborne, Daniel T, Prince, Jessica, Scully, Eileen, Macharia, Gladys, Micci, Luca, Lawson, Benton, Kopycinski, Jakub, Deymier, Martin J, Vanderford, Thomas H, Nganou-Makamdop, Krystell, Ende, Zachary, Brooks, Kelsie, Tang, Jianming, Yu, Tianwei, Lakhi, Shabir, Kilembe, William, Silvestri, Guido, Douek, Daniel, Goepfert, Paul A, Price, Matthew, Allen, Susan A, Paiardini, Mirko, Altfeld, Marcus, Gilmour, Jill, Hunter, Eric
    Proc Natl Acad Sci U S A, 2015-02-17;112(12):E1480-9.
    Species: Human
    Sample Types: Plasma
  28. Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation.
    Authors: Schuetz A, Deleage C, Sereti I, Rerknimitr R, Phanuphak N, Phuang-Ngern Y, Estes J, Sandler N, Sukhumvittaya S, Marovich M, Jongrakthaitae S, Akapirat S, Fletscher J, Kroon E, Dewar R, Trichavaroj R, Chomchey N, Douek D, O Connell R, Ngauy V, Robb M, Phanuphak P, Michael N, Excler J, Kim J, de Souza M, Ananworanich J
    PLoS Pathog, 2014-12-11;10(12):e1004543.
    Species: Human
    Sample Types: Plasma

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Reviews for Human FABP2/I-FABP DuoSet ELISA

Average Rating: 4.3 (Based on 3 Reviews)

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Human FABP2/I-FABP DuoSet ELISA
By Anonymous on 04/02/2020
Sample Tested: Serum and Plasma

We used this kit for the quantification of I-FABP in human serum and plasma. Works very well and well-described protocol.

Human FABP2/I-FABP DuoSet ELISA DY3078
Human FABP2/I-FABP DuoSet ELISA
By Jonatan Dereke on 03/07/2019
Sample Tested: Serum

We run human serum samples at a 1:5 dilution for this assay with high precision.

Human FABP2/I-FABP DuoSet ELISA DY3078
Human FABP2/I-FABP DuoSet ELISA
By Anonymous on 12/11/2018
Sample Tested: Serum and Plasma
Human FABP2/I-FABP DuoSet ELISA DY3078