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Human FABP6 Antibody

Catalog #: AF3880
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3880
AF3880-SP
FABP6 in T84 Human Cell Line.
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Citations (2)
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Human FABP6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP7, and recombinant mouse FABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP6
Ala2-Ala128
Accession # P51161
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FABP6
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry FABP6 antibody in T84 Human Cell Line by Immunocytochemistry (ICC). View Larger

FABP6 in T84 Human Cell Line. FABP6 was detected in immersion fixed T84 human colon carcinoma cell line using Goat Anti-Human FABP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP6

Fatty acid binding protein-6 (FABP6), also known as ileal bile acid binding protein (I-BABP) and gastrotropin, is a 15 kDa cytoplasmic protein that belongs to the FABP family. It is expressed in ileal epithelium and multiple other tissues. Alternate transcription promoters generate two transcript variants, encoding a 128 aa and a 177 aa residue protein. Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. It binds both fatty acids and bile acids and has roles in fatty acid transport and metabolism. The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively.

Long Name
Fatty Acid-Binding Protein 6
Entrez Gene IDs
2172 (Human); 16204 (Mouse); 25440 (Rat)
Alternate Names
FABP6; fatty acid binding protein 6, ileal; Fatty acid-binding protein 6; Gastrotropin; I-15P; I-15PIntestinal 15 kDa protein; IBABP; I-BABPIntestinal bile acid-binding protein; I-BALB; I-BAP; ILBP; ILBP3; ILBPGT; ileal bile acid binding protein; ILLBP; ILLBPIleal lipid-binding protein; illeal lipid-binding protein

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Citations for Human FABP6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling
    Authors: W Gu, H Wang, X Huang, J Kraiczy, PNP Singh, C Ng, S Dagdeviren, S Houghton, O Pellon-Car, Y Lan, Y Nie, J Zhang, KK Banerjee, EJ Onufer, BW Warner, J Spence, E Scherl, S Rafii, RT Lee, MP Verzi, D Redmond, R Longman, K Helin, RA Shivdasani, Q Zhou
    Cell Stem Cell, 2021-09-27;0(0):.
  2. Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels
    Authors: Claudia Loebel, Robert L. Mauck, Jason A. Burdick
    Nature Materials

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