Human FABP6 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3880
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Human FABP6 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human FABP1, 2, 3, 4, 5, 7, and recombinant mouse FABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP6
Ala2-Ala128
Accession # P51161
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FABP6
Immunocytochemistry
5-15 µg/mL
Immersion fixed T84 human colon carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP6

Fatty acid binding protein-6 (FABP6), also known as ileal bile acid binding protein (I-BABP) and gastrotropin, is a 15 kDa cytoplasmic protein that belongs to the FABP family. It is expressed in ileal epithelium and multiple other tissues. Alternate transcription promoters generate two transcript variants, encoding a 128 aa and a 177 aa residue protein. Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. It binds both fatty acids and bile acids and has roles in fatty acid transport and metabolism. The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively.

Long Name
Fatty Acid-Binding Protein 6
Entrez Gene IDs
2172 (Human); 16204 (Mouse); 25440 (Rat)
Alternate Names
FABP6; fatty acid binding protein 6, ileal; Fatty acid-binding protein 6; Gastrotropin; I-15P; I-15PIntestinal 15 kDa protein; IBABP; I-BABPIntestinal bile acid-binding protein; I-BALB; I-BAP; ILBP; ILBP3; ILBPGT; ileal bile acid binding protein; ILLBP; ILLBPIleal lipid-binding protein; illeal lipid-binding protein

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