Human FABP9/T-FABP Antibody

Catalog #: AF5707 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5707
AF5707-SP

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Human FABP9/T-FABP Antibody Summary

Species Reactivity

Human

Specificity

Detects human FABP9 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse FABP9 and less than 1% cross-reactivity with recombinant human FABP1, 2, 3, 4, 5, 6, 7, and 8 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FABP9
Met1-Val132
Accession # Q0Z7S8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant human FABP9

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FABP9/T‑FABP in Human Testis. FABP9/T-FABP was detected in immersion fixed paraffin-embedded sections of human testis using Human FABP9/T-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5707) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP9/T-FABP

FABP9 (fatty acid binding protein-9; also T-FABP and PERF15) is a cytosolic 15 kDa member of the fatty acid binding protein family, calycin superfamily of proteins. It is found in midpachytene spermatocytes and round spermatids, and constitutes part of the perinuclear theca. Functionally, FABP9 is likely to link intracellular membranes, and may signal abnormal sperm formation during spermatogenesis. Human FABP9 is 132 amino acids (aa) in length and exhibits two layers of antiparallel beta -strands that envelope a hydrophobic pocket for lipid binding. Human FABP9 shares 71% aa identity with mouse FABP9.

Long Name

Fatty Acid-Binding Protein 9

Entrez Gene IDs

646480 (Human); 21884 (Mouse)

Alternate Names

FABP9; FABP-9; Fatty Acid Binding Protein 9, Testis; Fatty Acid-Binding Protein 9; Lipid-Binding Protein; PERF 15; PERF; PERF15; Testis Lipid Binding Protein; Testis Lipid-Binding Protein; Testis-Type Fatty Acid-Binding Protein; TFABP; T-FABP; TLBP