Human FAM20B Antibody

Catalog #: MAB84272 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB84272-100
MAB84272-SP

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Human FAM20B Antibody Summary

Species Reactivity

Human

Specificity

Detects human FAM20B in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1018569

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FAM20B
Asn27-Leu409
Accession # O75063

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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FAM20B in SH‑SY5Y Human Cell Line. FAM20B was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human FAM20B Monoclonal Antibody (Catalog # MAB84272) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM20B

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

References

Nalbant, D. et al. (2005) BMC Genomics 6:11.
Koike, T. et al. (2009) Biochem. J. 421:157.
Vogel, P. et al. (2012) Vet. Pathol. 49:998.
Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
Wu, Z.L. (2011) PLoS One 6:e23172.

Long Name

Family with Sequence Similarity 20, Member B

Entrez Gene IDs

9917 (Human); 215015 (Mouse); 304885 (Rat)

Alternate Names

EC 2.7.1.-; FAM20B; family with sequence similarity 20, member B; Glycosaminoglycan Xylosylkinase; gxk1; KIAA0475; Protein FAM20B; XYLK; Xylose Kinase