Human FAP QuicKit ELISA New

Catalog #: QK3715 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
QK3715
SK3715
PK3715

Control Products Available

QC313: QuicKit Immunoassay Control Set 1198 for Human FAP

Human FAP Quantikine QuicKit ELISA Standard Curve

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All Fibroblast Activation Protein alpha/FAP ELISAs

Product Details

Supplemental Products

Reviews

Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human FAP QuicKit ELISA Summary

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)

Sensitivity

9.01 pg/mL

Assay Range

54.7 - 3,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human FAP.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine QuicKit Human FAP Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human FAP levels in cell culture supernates, serum, and plasma. It contains recombinant human FAP and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FAP showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural FAP.

Recovery

The recovery of human FAP spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	94-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FAP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human FAP Quantikine QuicKit ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibroblast Activation Protein alpha/FAP

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Entrez Gene IDs:

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Alternate Names:

170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin