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Human Fas Ligand/TNFSF6 ELISA Kit - Quantikine

Catalog #: DFL00B
29 Citations Datasheet / COA / SDS
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DFL00B
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QC89: Quantikine Immunoassay Control Set 402 for Human Fas Ligand
Human Fas Ligand/TNFSF6 Standard Curve
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Citations (29)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Fas Ligand/TNFSF6 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
5.1 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Fas Ligand.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Fas Ligand/TNFSF6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Fas Ligand.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 92.1 240 587 98.6 244 576
Standard Deviation 3.31 7.51 18.2 4.41 8.7 25.7
CV% 3.6 3.1 3.1 4.5 3.6 4.5

Recovery

The recovery of human Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 98 93-103
EDTA Plasma (n=4) 103 94-111
Heparin Plasma (n=4) 96 92-101
Serum (n=4) 101 96-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Fas Ligand were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Fas Ligand/TNFSF6 ELISA Linearity
Human Fas Ligand/TNFSF6 ELISA Linearity

Scientific Data

N/A Human Fas Ligand/TNFSF6 Standard Curve View Larger

Human Fas Ligand/TNFSF6 Standard Curve

N/A Human Fas Ligand/TNFSF6 ELISA Standard Curve View Larger

Human Fas Ligand/TNFSF6 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fas Ligand/TNFSF6

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

Entrez Gene IDs:
356 (Human); 14103 (Mouse); 25385 (Rat)
Alternate Names:
apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Possible link of genetic variants to autoimmunity in GAD-antibody-associated neurological disorders
    Authors: FS Thaler, B Bangol, M Biljecki, J Havla, AM Schumacher, T Kümpfel
    J. Neurol. Sci., 2020-04-29;413(0):116860.
    Species: Human
    Sample Types: Serum
  2. Efficient recruitment of c-FLIPL to the death-inducing signaling complex leads to Fas resistance in natural killer-cell lymphoma
    Authors: A Masuda, Y Isobe, K Sugimoto, M Yoshimori, A Arai, N Komatsu
    Cancer Sci., 2020-01-31;111(3):807-816.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Tumor necrosis factor-alpha and Fas/Fas ligand signaling pathways in chronic spontaneous urticaria
    Authors: R Grzanka, A Damasiewic, A Kasperska-
    Allergy Asthma Clin Immunol, 2019-03-14;15(0):15.
    Species: Human
    Sample Types: Serum
  4. Host Immunological Effects of Partial Splenic Embolization in Patients with Liver Cirrhosis
    Authors: Y Matsukiyo, H Nagai, T Matsui, Y Igarashi
    J Immunol Res, 2018-07-15;2018(0):1746391.
    Species: Human
    Sample Types: Serum
  5. Evaluation of soluble concentration Fas and Fas ligand in maternal and cord blood 3rd trimester of pregnancy
    Authors: A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu
    Ginekol. Pol., 2018-01-01;89(3):142-146.
    Species: Human
    Sample Types: Serum
  6. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
    Sci Rep, 2016-11-18;6(0):36862.
    Species: Human
    Sample Types: Plasma
  7. SAP facilitates recruitment and activation of LCK at NTB-A receptors during restimulation-induced cell death.
    Authors: Katz, Gil, Krummey, Scott M, Larsen, Sasha E, Stinson, Jeffrey, Snow, Andrew L
    J Immunol, 2014-03-31;192(9):4202-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation.
    Authors: Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X
    J Biol Chem, 2014-03-13;289(16):11522-35.
    Species: Mouse
    Sample Types: Whole Cells
  9. Natural history of autoimmune lymphoproliferative syndrome associated with FAS gene mutations.
    Authors: Price S, Shaw P, Seitz A, Joshi G, Davis J, Niemela J, Perkins K, Hornung R, Folio L, Rosenberg P, Puck J, Hsu A, Lo B, Pittaluga S, Jaffe E, Fleisher T, Rao V, Lenardo M
    Blood, 2014-01-07;123(13):1989-99.
    Species: Human
    Sample Types: Serum
  10. Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
    Authors: Beynon V, Quintana FJ, Weiner HL
    PLoS ONE, 2012-06-29;7(6):e39576.
    Species: Human
    Sample Types: Serum
  11. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 2011-12-21;5(2):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Impaired thymic export and apoptosis contribute to regulatory T-cell defects in patients with chronic heart failure.
    Authors: Tang TT, Zhu ZF, Wang J, Zhang WC, Tu X, Xiao H, Du XL, Xia JH, Dong NG, Su W, Xia N, Yan XX, Nie SF, Liu J, Zhou SF, Yao R, Xie JJ, Jevallee H, Wang X, Liao MY, Shi GP, Fu M, Liao YH, Cheng X
    PLoS ONE, 2011-09-15;6(9):e24272.
    Species: Human
    Sample Types: Plasma
  13. Somatic FAS mutations are common in patients with genetically undefined autoimmune lymphoproliferative syndrome.
    Authors: Dowdell KC, Niemela JE, Price S, Davis J, Hornung RL, Oliveira JB, Puck JM, Jaffe ES, Pittaluga S, Cohen JI, Fleisher TA, Rao VK
    Blood, 2010-04-01;115(25):5164-9.
    Species: Human
    Sample Types: Plasma
  14. Effect of quinapril on in-stent restenosis and relation to plasma apoptosis signaling molecules.
    Authors: Deftereos S, Giannopoulos G, Kossyvakis C, Kaoukis A, Raisakis K, Driva M, Panagopoulou V, Lappos S, Rentoukas I, Pyrgakis V, Alpert MA
    Am. J. Cardiol., 2009-11-14;105(1):54-8.
    Species: Human
    Sample Types: Plasma
  15. A 60-s postconditioning protocol by percutaneous coronary intervention inhibits myocardial apoptosis in patients with acute myocardial infarction.
    Authors: Zhao WS, Xu L, Wang LF, Zhang L, Zhang ZY, Liu Y, Liu XL, Yang XC, Cui L, Zhang L
    Apoptosis, 2009-10-01;14(10):1204-11.
    Species: Human
    Sample Types: Plasma
  16. Differential effects of arsenic on cutaneous and systemic immunity: focusing on CD4+ cell apoptosis in patients with arsenic-induced Bowen's disease.
    Authors: Liao WT, Yu CL, Lan CC, Lee CH, Chang CH, Chang LW, You HL, Yu HS
    Carcinogenesis, 2009-04-17;30(6):1064-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. The influence of hepatic damage on serum soluble Fas ligand levels of patients with drug rashes.
    Authors: Tohyama M, Shirakata Y, Sayama K, Hashimoto K
    J. Allergy Clin. Immunol., 2009-04-01;123(4):971-2; author.
    Species: Human
    Sample Types: Serum
  18. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009-03-16;206(4):793-805.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis.
    Authors: Chung WH, Hung SI, Yang JY, Su SC, Huang SP, Wei CY, Chin SW, Chiou CC, Chu SC, Ho HC, Yang CH, Lu CF, Wu JY, Liao YD, Chen YT
    Nat. Med., 2008-11-23;14(12):1343-50.
    Species: Human
    Sample Types: Blister Fluid
  20. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure.
    Authors: Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT
    Atherosclerosis, 2007-11-07;199(1):215-21.
    Species: Human
    Sample Types: Plasma
  22. Functional polymorphisms in FAS and FASL contribute to increased apoptosis of tumor infiltration lymphocytes and risk of breast cancer.
    Authors: Zhang B, Sun T, Xue L, Han X, Zhang B, Lu N, Shi Y, Tan W, Zhou Y, Zhao D, Zhang X, Guo Y, Lin D
    Carcinogenesis, 2006-12-20;28(5):1067-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism.
    Authors: Harris LK, Keogh RJ, Wareing M, Baker PN, Cartwright JE, Aplin JD, Whitley GS
    Am. J. Pathol., 2006-11-01;169(5):1863-74.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
    Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
    Am. J. Respir. Crit. Care Med., 2006-03-30;174(1):51-7.
    Species: Human
    Sample Types: Plasma
  25. Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy.
    Authors: Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D
    Eur J Cardiovasc Prev Rehabil, 2005-04-01;12(2):164-8.
    Species: Human
    Sample Types: Plasma
  26. Vitamin C inhibits platelet expression of CD40 ligand.
    Authors: Pignatelli P, Sanguigni V, Paola SG, Lo Coco E, Lenti L, Violi F
    Free Radic. Biol. Med., 2005-03-23;38(12):1662-6.
    Species: Human
    Sample Types: Plasma
  27. High-dose glucose-insulin-potassium treatment reduces myocardial apoptosis in patients with acute myocardial infarction.
    Authors: Zhang L, Li YH, Zhang HY, Chen ML, Gao MM, Hu AH, Yang HS
    Eur. J. Clin. Invest., 2005-03-01;35(3):164-70.
    Species: Human
    Sample Types: Plasma
  28. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
    Authors: Alter P, Maisch B
    Z Kardiol, 2004-07-01;93(7):524-32.
    Species: Human
    Sample Types: Serum
  29. Wheat gliadin induces apoptosis of intestinal cells via an autocrine mechanism involving Fas-Fas ligand pathway.
    Authors: D&amp;apos;Archivio M
    FEBS Lett., 2003-04-10;540(1):117-24.
    Species: Human
    Sample Types: Cell Culture Supernates

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