Human FCRLB/FCRY Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% amino acid sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1‑3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1‑3). Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig-like domain (3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (3, 4). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (3, 4). It is upregulated in B cells by BAFF and LPS (5). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 5). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (4, 5).

Product Datasheets

Related Research Areas

• B Cell Activation
• Fc Receptors