Human/Feline IL‑23 p19 Antibody

Catalog #: AF6238 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6238
AF6238-SP

Detection of Human IL‑23 p19 by Western Blot.

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Human/Feline IL‑23 p19 Antibody Summary

Species Reactivity

Human, Feline

Specificity

Detects human and feline IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant canine IL-23 p19 is observed and approximately 30% cross-reactivity with recombinant mouse IL-23 p19 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant feline IL-23 p19
Arg26-Arg185
Accession # ABB01676

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑23 p19 by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Feline IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-23 p19 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

IL-23 p19 (Interleukin 23 p19; also IL-23 alpha) is an 18-20 kDa (estimated) member of the IL-6 superfamily of molecules. It is disulfide-bonded to p40 to form IL-23. IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation. It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-gamma production by CD4+, CD8+ and NK cells. Based on human, mature feline IL-23 p19 is 160 amino acids (aa) in length. It is a alpha -helical molecule that utilizes Cys80 to form an interchain disulfide bond with IL-12 p40. IL-23 p19 does not appear to be released unless dimerized to p40. There are no potential N-linked glycosylation sites on p19. Mature feline IL-23 p19 (aa 26-185) shares 87% aa identity with human IL-23 p19.

Long Name

Interleukin 23

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Alternate Names

IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha