Human FGF-12 Antibody

Catalog #: AF2246 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2246
AF2246-SP

All FGF-12 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-12 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) FGF-13B is observed, 10% cross-reactivity with rhFGF-11 is observed and less than 1% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-3, rhFGF-4, rhFGF-6, rhFGF-7, rhFGF-9, rhFGF-10, rhFGF-16, rhFGF-17, rhFGF-18, rhFGF-19, rhFGF-20, rhFGF-23, recombinant mouse (rm) FGF-15, and rmFGF-8b is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FGF-12
Glu67-Thr243
Accession # P61328

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FGF‑12 (Catalog # 2246-FG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-12

Fibroblast growth factor 12 (FGF-12) is a member of the FGF superfamily of molecules which contains at least 22 members. The FGFs are characterized by the presence of a core, 120 amino acid (aa) beta -trefoil structure, and all apparently bind heparin (1, 2). FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, -4, respectively, form a subgroup within the FGF family (1, 3). Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered to be a cytoplasmic protein. It does, however, possess an N-terminal bipartite nuclear localization signal (NLS) at aa 11‑18 and 28‑38 (4‑6). The 243 aa protein has at least one alternate splice form that is 181 aa in length. This is termed FGF-12B. Alternate splicing deletes the N-terminal 66 aa in FGF‑12 and replaces them with four aa in FGF-12B. This substitution removes the NLS from the short form. Studies on the short form (12B) show that it cannot bind any of the common FGF receptors. This is consistent with its cytoplasmic localization. It can, however, bind to IB2 (islet brain-2), a cellular kinase scaffold protein, and voltage-gated sodium channels, suggesting FGF-12B plays an important role in intracellular signaling and ion exchange (7). Mouse and human FGF-12B differ by only one amino acid.

References

Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
Ornitz, D.M. and N. Itoh (2001) Genome Biol. 2:REVIEWS 3005.
Green, P. et al. (1996) BioEssays 18:639.
Smallwood, P. M. et al. (1996) Proc. Natl. Acad. Sci. 93:9850.
Munoz-Sanjuan, I. et al. (2000) J. Biol. Chem 275:2589.
Kok, L.D.S. et al. (1999) Biochem. Biophys. Res. Commun. 255:717.
Olsen, S. K. et al. (2003) J. Biol. Chem. 278:34226.

Long Name

Fibroblast Growth Factor 12

Entrez Gene IDs

2257 (Human)

Alternate Names

FGF12; FGF-12; FGF12B; FHF1; FHF-1; FHF1Fibroblast growth factor homologous factor 1; fibroblast growth factor 12; fibroblast growth factor 12B; fibroblast growth factor FGF-12b; Myocyte-activating factor