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Human FGF-19 Antibody

Catalog #: MAB969
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB969-SP
MAB969-500
MAB969-100
FGF‑19 in Human Prostate Cancer Tissue.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-19 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-19 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF‑4, -5, -6, -7, -9, -10, -17, -18, recombinant mouse FGF-8b, -8c, or -15 is observed.
Source
Monoclonal Mouse IgG1 Clone # 117601
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FGF-19
Phe27-Lys216
Accession # O95750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF‑19 (Catalog # 969-FG)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FGF-19 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

FGF‑19 in Human Prostate Cancer Tissue.

FGF-19 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human FGF-19 Monoclonal Antibody (Catalog # MAB969) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-19

Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5).

References
  1. Nishimura, T. et al. (1999) Biocheim. Biophys. Acta 1444:148.
  2. Xie, M. et al. (1999) Cytokine 11:729.
  3. Goldfarb, M. (1996) Cytokine & Growth Factor Reviews 7:311.
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Ladher, R.K. et al. (2000) Science 290:1965.
Long Name
Fibroblast Growth Factor 19
Entrez Gene IDs
9965 (Human)
Alternate Names
FGF19; FGF-19; fibroblast growth factor 19

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Citations for Human FGF-19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expression of hepatic Fibroblast Growth Factor 19 is enhanced in Primary Biliary Cirrhosis and correlates with severity of the disease
    Authors: Ewa Wunsch, Małgorzata Milkiewicz, Urszula Wasik, Jocelyn Trottier, Agnieszka Kempińska-Podhorodecka, Elwyn Elias et al.
    Scientific Reports
  2. Impaired Hepatic Adaptation to Chronic Cholestasis induced by Primary Sclerosing Cholangitis
    Sci Rep, 2016-12-23;6(0):39573.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Endocrine fibroblast growth factor FGF19 promotes prostate cancer progression.
    Authors: Feng S, Dakhova O, Creighton C, Ittmann M
    Cancer Res, 2013-02-25;73(8):2551-62.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Tissue-specific expression of betaKlotho and fibroblast growth factor (FGF) receptor isoforms determines metabolic activity of FGF19 and FGF21.
    Authors: Kurosu H, Choi M, Ogawa Y, Dickson AS, Goetz R, Eliseenkova AV, Mohammadi M, Rosenblatt KP, Kliewer SA, Kuro-o M
    J. Biol. Chem., 2007-07-10;282(37):26687-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human FGF-19 Antibody
By Anonymous on 02/11/2022
Application: IHC Sample Tested: Prostate cancer tissue Species: Human
Immunohistochemistry Human FGF-19 Antibody MAB969