Home / FGF-20 / Human FGF-20 Antibody

Human FGF-20 Antibody

Catalog #: AF2547
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2547
AF2547-SP
Cell Proliferation Induced by FGF‑20 and Neutralization by Human FGF‑20 Antibody.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-20 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF‑20 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human (rh) FGF-16 and rhFGF-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FGF-20
Pro3-Thr211
Accession # Q9NP95
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FGF‑20 (Catalog # 2547-FG)
Neutralization
Measured by its ability to neutralize FGF‑20-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.05-0.35 µg/mL in the presence of 10 ng/mL Recombinant Human FGF‑20 and 1 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF‑20 and Neutralization by Human FGF‑20 Antibody. View Larger

Cell Proliferation Induced by FGF‑20 and Neutralization by Human FGF‑20 Antibody. Recombinant Human FGF-20 (Catalog # 2547-FG) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2547). The ND50 is typically 0.05-0.35 µg/mL in the presence of heparin (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-20

Fibroblast growth factor 20 (FGF-20) is a member of the FGF gene family, which currently contains 22 members. Based on its structure, it is further classified as an FGF-9 subfamily member. All FGF family members are heparin-binding growth factors with a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1). The cDNA of FGF-20 predicts a 211 aa polypeptide without a canonical signal peptide sequence, a feature shared with other members of this subfamily (2‑4). Nevertheless, it is secreted with a molecular weight of 27 kDa (2‑4). FGF-20 is known to bind to heparin (4). No alternate splice forms have been reported. However, three amino acid polymorphisms are known, and single nucleotide polymorphisms in noncoding regions that may effect expression show a strong correlation with a risk of developing Parkinson’s disease (5, 6). Human FGF-20 shows 98% aa identity to bovine FGF-20 and 95% aa identity to both rat and mouse FGF-20. Within the FGF-9 subfamily, FGF-20 is 69% and 63% aa identical to human FGF-9 and FGF-16, respectively. Human FGF-20 is reported to be promiscuous in its selection of receptors which include FGF R1c, FGF R2c, FGF R3b, FGF R3c and FGF R4 (4, 7, 8). FGF-20 is expressed a variety of cells, including dopaminergic neurons (2), fibroblasts, keratinocytes and breast epithelium (4), and multiple sites in the fetus (2, 7). Finally, the expression of FGF-20 and DKK-1 is regulated by beta -catenin during development and tumorigenesis, implying that FGF-20 may play a role in the oncogenesis induced by the Wnt signaling pathway (9).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Ohmachi, S. et al. (2000) Biochem. Biophys. Res. Commun. 277:355.
  3. Kirikoshi, H. et al. (2000) Biochem. Biophys. Res. Commun. 274:337.
  4. Jeffers, M. et al. (2001) Cancer Res. 61:3131.
  5. van der Walt, J. et al. (2004) Am. J. Hum. Genet. 74:1121.
  6. Genbank Accession # Q9NP95.
  7. Lavine, K.J. et al. (2005) Dev. Cell 8:85.
  8. Ohmachi, S. et al. (2003) J. Neurosci. Res. 72:436.
  9. Chamorro, M.N. et al. (2005) EMBO J. 24:73.
Long Name
Fibroblast Growth Factor 20
Entrez Gene IDs
26281 (Human)
Alternate Names
FGF20; FGF-20; fibroblast growth factor 20

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human FGF-20 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fgf20 is required for sensory epithelial specification in the developing cochlea.
    Authors: Hayashi T, Ray CA, Bermingham-McDonogh O
    J. Neurosci., 2008-06-04;28(23):5991-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FGF-20 Antibody

There are currently no reviews for this product. Be the first to review Human FGF-20 Antibody and earn rewards!

Have you used Human FGF-20 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review