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Human FGF-21 Antibody

Catalog #: MAB25373 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB25373-SP
MAB25373-100
Cell Proliferation Induced by FGF-21 and Neutralization by Human FGF-21 Antibody.
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Human FGF-21 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-21 in direct ELISAs.
Source
Recombinant Monoclonal Goat IgG2B Clone # 40020A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human FGF-21
His29-Ser209
Accession # Q9NSA1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize FGF-21-induced proliferation in BaF3 mouse pro-B cell line transfected with human FGF-RIIIc. The Neutralization Dose (ND50) is typically 0.5-3 μg/mL in the presence of Recombinant Mouse Klotho beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF-21 and Neutralization by Human FGF-21 Antibody. View Larger

Cell Proliferation Induced by FGF-21 and Neutralization by Human FGF-21 Antibody. In the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB), Recombinant Human FGF-21 (Catalog # 2539-FG) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human FGF-RIIIc in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-21 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-21 Mononclonal Antibody (MAB25373). The ND50 is typically 0.5-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitue at 0.5mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-21

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the
C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6).
The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a
high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Huang, X. et al. (2006) Mol. Carcinog. 45:934.
  4. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
  5. GenBank Accession #: EAW52401 (2006).
  6. Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627.
  7. Moore, D. D. (2007) Science 316:1436.
  8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  9. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
  10. Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437.
  11. Badman, M.K. et al. (2007) Cell Metab. 5:426.
  12. Inagaki, T. et al. (2007) Cell Metab. 5:415.
Long Name
Fibroblast Growth Factor 21
Entrez Gene IDs
26291 (Human); 56636 (Mouse)
Alternate Names
FGF21; FGF-21; fibroblast growth factor 21

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