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Human FGF-21 ELISA Kit - Quantikine

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Human FGF-21 ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human FGF-21 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
8.69 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human FGF-21
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human FGF-21 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=35)17297ND-914
EDTA plasma (n=35)20197ND-1155
Heparin plasma (n=35)18697ND-1012
ND=Non-detectable

Cell Culture Supernates - Human peripheral blood leukocytes (PBLs) were cultured in DMEM supplemented with 5% fetal bovine serum, 5 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for human FGF-21. No detectable levels were observed.

Product Summary

The Quantikine Human FGF-21 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-21 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-21 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-21 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-21.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 204 672 1324 245 742 1411
Standard Deviation 8 19.8 46.2 26.6 47.2 73.9
CV% 3.9 2.9 3.5 10.9 6.4 5.2

Recovery

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-108
EDTA Plasma (n=4) 103 92-112
Heparin Plasma (n=4) 98 83-111
Serum (n=4) 105 87-119

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FGF-21 ELISA Linearity

Scientific Data

N/A Human FGF-21 ELISA Standard Curve View Larger

Human FGF-21 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-21

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which contains 22 mammalian members. Based on its structure, it is further classified as a member of the FGF-19 subfamily, which also includes FGF-19 and FGF-23 (1-4). FGF family members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure. FGF-19 subfamily members, unlike other FGFs, lack one strand of the beta -trefoil and bind poorly to extracellular matrix molecules such as heparin (3). They are consequently more diffusible than other FGFs and are considered endocrine rather than paracrine (1-4). All three subfamily members impact some aspect of metabolism; all three are induced by a nuclear receptor heterodimer that includes RXR (retinoid X receptor), and all three bind FGF receptors (FGF R) indirectly through co-receptors of the klotho family (5-9). FGF-21 binds to beta -Klotho via its C-terminal sequence. This binding, along with amino acids at the N-terminus, is required for signaling through FGF R (7, 8). FGF-21 is selective for FGF R1 isoform 1c, with varying reports of using isoforms 2c or 3c (10-12). Presence of the required klotho and FGF R family members determines tissue specificity of FGF-19 subfamily members, and thus concentrates FGF-21 activity within adipose tissue (3, 9-11). Mature human FGF-21 shares 81% aa sequence identity with mouse and rat FGF-21. 

FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPAR alpha /RXR dimeric complex (4, 13-15). This situation occurs during starvation, diabetic ketosis, or following the ingestion of a high-fat/low-carbohydrate or ketogenic diet (5, 14-16). Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach the hepatocytes, they are oxidized and reduced to acetyl-CoA (16). The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for energy generation (5, 15, 16). FGF-21 production is also induced upon differentiation of human or mouse fibroblasts to adipocytes (17, 18). In adipose tissue, FGF-21 induces glucose uptake by signaling in synergy with PPAR gamma to increase production of the glucose transporter, GLUT1 (10, 12, 19). 
FGF-21 production follows a circadian pattern in mice (20). It diffuses across the blood-brain barrier and this may facilitate induction of a state of torpor, or decreased activity, in response to increased FGF-21 (16, 21). These characteristics appear to induce a hibernation-like state during fasting and short days in winter (22). In diet-induced obese mice and mouse models of diabetes such as db/db and ob/ob, administration or transgenic overexpression of FGF-21 restores circulating glucose and triglyceride values to near normal and increases insulin sensitivity (5, 6, 14, 23, 24). In some of these states and in human obesity and type II diabetes, FGF-21 is already elevated prior to treatment, suggesting that resistance to FGF-21 is possible (17, 25, 26). Although FGF-21 administration corrects obesity in mice, it is unclear whether the same benefit would be seen in humans (2, 3, 17, 26-28).

Long Name:
Fibroblast Growth Factor 21
Entrez Gene IDs:
26291 (Human); 56636 (Mouse)
Alternate Names:
FGF21; FGF-21; fibroblast growth factor 21
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human FGF-21 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

55 Citations: Showing 1 - 10
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  1. FGF21 upregulation by hepatitis C virus via the eIF2?-ATF4 pathway: implications for interferon signaling suppression and TRIM31-mediated TSC degradation
    Authors: Liu, L;Ito, M;Sakai, S;Liu, J;Ohta, K;Saito, K;Nakashima, K;Satoh, S;Konno, A;Suzuki, T;
    Frontiers in microbiology
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Rapid benefits in older age from transition to whole food diet regardless of protein source or fat to carbohydrate ratio: Arandomised control trial
    Authors: Ribeiro, RV;Senior, AM;Simpson, SJ;Tan, J;Raubenheimer, D;Le Couteur, D;Macia, L;Holmes, A;Eberhard, J;O'Sullivan, J;Koay, YC;Kanjrawi, A;Yang, J;Kim, T;Gosby, A;
    Aging cell
    Species: Human
    Sample Types: Plasma
  3. Skin advanced glycation end-products as indicators of the metabolic profile in diabetes mellitus: correlations with glycemic control, liver phenotypes and metabolic biomarkers
    Authors: Christidis, G;Küppers, F;Karatayli, SC;Karatayli, E;Weber, SN;Lammert, F;Krawczyk, M;
    BMC endocrine disorders
    Species: Human
    Sample Types: Serum
  4. Intellectual disability and autism in propionic acidemia: a biomarker-behavioral investigation implicating dysregulated mitochondrial biology
    Authors: Shchelochkov, OA;Farmer, CA;Chlebowski, C;Adedipe, D;Ferry, S;Manoli, I;Pass, A;McCoy, S;Van Ryzin, C;Sloan, J;Thurm, A;Venditti, CP;
    Molecular psychiatry
    Species: Human
    Sample Types: Plasma
  5. Fibroblast Growth Factor 21 in Chronic Hepatitis C: A Potential Non-Invasive Biomarker of Liver Status upon Viral Eradication
    Authors: Biagi, F;Carlomagno, F;Carbone, M;Veralli, R;Vespasiani-Gentilucci, U;Riva, E;Manfrini, S;Tuccinardi, D;De Santis, A;Gnessi, L;Watanabe, M;
    Metabolites
    Species: Human
    Sample Types: Whole Blood
  6. Plasma Cytokeratin-18 Fragment Level Reflects the Metabolic Phenotype in Obesity
    Authors: Goralska, J;Razny, U;Gruca, A;Zdzienicka, A;Micek, A;Dembinska-Kiec, A;Solnica, B;Malczewska-Malec, M;
    Biomolecules
    Species: Human
    Sample Types: Plasma
  7. An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction
    Authors: JY Sun, LJ Du, XR Shi, YY Zhang, Y Liu, YL Wang, BY Chen, T Liu, H Zhu, Y Liu, CC Ruan, Z Gan, H Ying, Z Yin, PJ Gao, X Yan, RG Li, SZ Duan
    Science Advances, 2023-04-05;9(14):eade4110.
    Species: Human
    Sample Types: Plasma
  8. Novel Approach for Glycemic Management Incorporating Vibration Stimulation of Skeletal Muscle in Obesity
    Authors: M Kim, H Zhang, T Kim, Y Mori, T Okura, K Tanaka, T Isobe, T Sakae, S Oh
    International Journal of Environmental Research and Public Health, 2023-03-07;20(6):.
    Species: Human
    Sample Types: Serum
  9. Fibrosis of Peritoneal Membrane, Molecular Indicators of Aging and Frailty Unveil Vulnerable Patients in Long-Term Peritoneal Dialysis
    Authors: P Branco, R Calça, AR Martins, C Mateus, MJ Jervis, DP Gomes, S Azeredo-Lo, AF De Melo Ju, C Sousa, E Civantos, S Mas-Fontao, A Gaspar, S Ramos, J Morello, F Nolasco, A Rodrigues, SA Pereira
    International Journal of Molecular Sciences, 2023-03-06;24(5):.
    Species: Human
    Sample Types: Serum
  10. Elevated serum FGF21 levels predict heart failure during hospitalization of STEMI patients after emergency percutaneous coronary intervention
    Authors: L Gu, W Jiang, W Jiang, Z Xu, W Li, H Zhang
    PeerJ, 2023-02-06;11(0):e14855.
    Species: Human
    Sample Types: Serum
  11. Discovering Biomarkers for Non-Alcoholic Steatohepatitis Patients with and without Hepatocellular Carcinoma Using Fecal Metaproteomics
    Authors: S Sydor, C Dandyk, J Schwerdt, P Manka, D Benndorf, T Lehmann, K Schallert, M Wolf, U Reichl, A Canbay, LP Bechmann, R Heyer
    International Journal of Molecular Sciences, 2022-08-09;23(16):.
    Species: Human
    Sample Types: Serum
  12. Maternal Fibroblast Growth Factor 21 Levels Decrease during Early Pregnancy in Normotensive Pregnant Women but Are Higher in Preeclamptic Women-A Longitudinal Study
    Authors: JD Buell-Acos, MF Garces, AJ Parada-Bañ, E Angel-Mull, MC Paez, J Eslava-Sch, F Escobar-Co, SA Caminos-Ce, E Lacunza, JP Castaño, R Nogueiras, C Dieguez, AI Ruiz-Parra, JE Caminos
    Cells, 2022-07-21;11(14):.
    Species: Human
    Sample Types: Serum
  13. Inhibition of Adipose Tissue Beiging by HIV Integrase Inhibitors, Dolutegravir and Bictegravir, Is Associated with Adipocyte Hypertrophy, Hypoxia, Elevated Fibrosis, and Insulin Resistance in Simian Adipose Tissue and Human Adipocytes
    Authors: K Ngono Ayis, J Gorwood, L Le Pelleti, C Bourgeois, C Beaupère, M Auclair, R Foresti, R Motterlini, M Atlan, A Barrail-Tr, R Le Grand, D Desjardins, B Fève, O Lambotte, J Capeau, V Béréziat, C Lagathu
    Cells, 2022-06-04;11(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. PET/MRI-Evaluated Activation of Brown Adipose Tissue via Cold Exposure Impacts Lipid Metabolism
    Authors: K Miniewska, K Maliszewsk, K Pietrowska, J Godzie?, ? ?abieniec, M Mojsak, A Kr?towski, M Ciborowski
    Metabolites, 2022-05-19;12(5):.
    Species: Human
    Sample Types: Serum
  15. Application of Mitochondrial and Oxidative Stress Biomarkers in the Evaluation of Neurocognitive Prognosis Following Acute Carbon Monoxide Poisoning
    Authors: YS Cha, JS Chang, H Kim, KS Park
    Metabolites, 2022-02-24;12(3):.
    Species: Human
    Sample Types: Serum
  16. Association of serum fibroblast growth factor 21 with diabetic complications and insulin dose in patients with type 1 diabetes mellitus
    Authors: H Taniguchi, S Nirengi, K Ishihara, N Sakane
    PLoS ONE, 2022-02-22;17(2):e0263774.
    Species: Human
    Sample Types: Serum
  17. Fibroblast Growth Factor 19 and Fibroblast Growth Factor 21 Regulation in Obese Diabetics, and Non-Alcoholic Fatty Liver Disease after Gastric Bypass
    Authors: JY Guo, HH Chen, WJ Lee, SC Chen, SD Lee, CY Chen
    Nutrients, 2022-02-02;14(3):.
    Species: Human
    Sample Types: Plasma
  18. A Potential Role for Bile Acid Signaling in Celiac Disease-Associated Fatty Liver
    Authors: P Manka, S Sydor, JM Schänzer-O, M Brandenbur, J Best, R Vilchez-Va, A Link, D Heider, S Brodesser, A Figge, A Jähnert, JD Coombes, FJ Cubero, A Kahraman, MS Kim, J Kälsch, S Kinner, KN Faber, H Moshage, G Gerken, WK Syn, A Canbay, LP Bechmann
    Metabolites, 2022-01-30;12(2):.
    Species: Human
    Sample Types: Serum
  19. Serum levels of the cold stress hormones FGF21 and GDF-15 after cardiac arrest in infants and children enrolled in single center therapeutic hypothermia clinical trials
    Authors: JR Herrmann, EL Fink, A Fabio, AK Au, RP Berger, K Janesko-Fe, RSB Clark, PM Kochanek, TC Jackson
    Resuscitation, 2021-11-22;0(0):.
    Species: Human
    Sample Types: Serum
  20. Serum FGF21 levels are altered by various factors including lifestyle behaviors in male subjects
    Authors: K Nakanishi, C Ishibashi, S Ide, R Yamamoto, M Nishida, I Nagatomo, T Moriyama, K Yamauchi-T
    Scientific Reports, 2021-11-19;11(1):22632.
    Species: Human
    Sample Types: Serum
  21. Effects of exenatide on urinary albumin in overweight/obese patients with T2DM: a randomized clinical trial
    Authors: C Kang, Q Qiao, Q Tong, Q Bai, C Huang, R Fan, H Wang, K Kaliannan, J Wang, J Xu
    Scientific Reports, 2021-10-08;11(1):20062.
    Species: Human
    Sample Types: Serum
  22. Blood cell respiration rates and mtDNA copy number: A promising tool for the diagnosis of mitochondrial disease
    Authors: M Alonso, C Zabala, S Mansilla, L De Brun, J Martínez, M Garau, G Rivas, C Acosta, D Lens, A Cerisola, M Graña, H Naya, R Puentes, L Spangenber, V Raggio, A Lemes, L Castro, C Quijano
    Mitochondrion, 2021-09-16;61(0):31-43.
    Species: Human
    Sample Types: Plasma
  23. Increased Fibroblast Growth Factor 21 (FGF21) Concentration in Early Second Trimester Amniotic Fluid and Its Association with Fetal Growth
    Authors: N Vrachnis, S Argyridis, D Vrachnis, N Antonakopo, G Valsamakis, C Iavazzo, D Zygouris, N Salakos, A Rodolakis, N Vlahos, G Mastorakos, P Drakakis, Z Iliodromit
    Metabolites, 2021-08-28;11(9):.
    Species: Human
    Sample Types: Amniotic Fluid
  24. A map of metabolic phenotypes in patients with myalgic encephalomyelitis/chronic fatigue syndrome
    Authors: F Hoel, A Hoel, IK Pettersen, IG Rekeland, K Risa, K Alme, K Sørland, A Fosså, K Lien, I Herder, HL Thürmer, ME Gotaas, C Schäfer, RK Berge, K Sommerfelt, HP Marti, O Dahl, O Mella, Ø Fluge, KJ Tronstad
    JCI Insight, 2021-08-23;6(16):.
    Species: Human
    Sample Types: Serum
  25. Myokine secretion following moderate-intensity endurance exercise under different environmental temperatures
    Authors: Y Tsuchiya, K Goto
    Cytokine, 2021-05-29;144(0):155553.
    Species: Human
    Sample Types: Plasma
  26. Beneficial effects of whole-body cryotherapy on glucose homeostasis and amino acid profile are associated with a reduced myostatin serum concentration
    Authors: M Koz?owska, J Kortas, M ?ychowska, J Antosiewic, K ?uczek, S Perego, G Lombardi, E Ziemann
    Scientific Reports, 2021-03-29;11(1):7097.
    Species: Human
    Sample Types: Serum
  27. The Effects of Bilirubin and Lumirubin on Metabolic and Oxidative Stress Markers
    Authors: A Dvo?ák, K Pospíšilov, K Žížalová, N Capková, L Muchová, M Vecka, N Vrzá?ková, J K?ížová, J Zelenka, L Vítek
    Frontiers in Pharmacology, 2021-03-04;12(0):567001.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Epigenetic Regulation of Processes Related to High Level of Fibroblast Growth Factor 21 in Obese Subjects
    Authors: T P?atek, A Polus, J Góralska, U Ra?ny, A Dziewo?ska, A Micek, A Dembi?ska-, B Solnica, M Malczewska
    Genes, 2021-02-21;12(2):.
    Species: Human
    Sample Types: Serum
  29. Role of PAI-1 in hepatic steatosis and dyslipidemia
    Authors: JA Levine, C Oleaga, M Eren, AP Amaral, M Shang, E Lux, SS Khan, SJ Shah, Y Omura, N Pamir, J Hay, G Barish, T Miyata, H Tavori, S Fazio, DE Vaughan
    Scientific Reports, 2021-01-11;11(1):430.
    Species: Human
    Sample Types: Plasma
  30. Mitochondrial dysfunction underlying sporadic inclusion body myositis is ameliorated by the mitochondrial homing drug MA-5
    Authors: Y Oikawa, R Izumi, M Koide, Y Hagiwara, M Kanzaki, N Suzuki, K Kikuchi, T Matsuhashi, Y Akiyama, M Ichijo, S Watanabe, T Toyohara, T Suzuki, E Mishima, Y Akiyama, Y Ogata, C Suzuki, H Hayashi, EN Kodama, KI Hayashi, E Itoi, M Aoki, S Kure, T Abe
    PLoS ONE, 2020-12-02;15(12):e0231064.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Hybrid Training System Improves Insulin Resistance in Patients with Nonalcoholic Fatty Liver Disease: A Randomized Controlled Pilot Study
    Authors: S Iwanaga, R Hashida, Y Takano, M Bekki, D Nakano, M Omoto, T Nago, T Kawaguchi, H Matsuse, T Torimura, N Shiba
    Tohoku J. Exp. Med., 2020-09-01;252(1):23-32.
    Species: Human
    Sample Types: Serum
  32. Growth Differentiation Factor 15 is a potential biomarker of therapeutic response for TK2 deficient myopathy
    Authors: C Dominguez-, C Badosa, M Madruga-Ga, I Martí, C Paradas, C Ortez, J Diaz-Maner, A Berardo, J Alonso-Pér, S Trifunov, D Cuadras, SG Kalko, C Blázquez-B, Y Cámara, R Martí, F Mavillard, MA Martin, J Montoya, E Ruiz-Pesin, J Villarroya, R Montero, F Villarroya, R Artuch, M Hirano, A Nascimento, C Jimenez-Ma
    Sci Rep, 2020-06-22;10(1):10111.
    Species: Human
    Sample Types: Serum
  33. Monounsaturated fat rapidly induces hepatic gluconeogenesis and whole-body insulin resistance
    Authors: T Sarabhai, S Kahl, J Szendroedi, DF Markgraf, OP Zaharia, C Barosa, C Herder, F Wickrath, P Bobrov, JH Hwang, JG Jones, M Roden
    JCI Insight, 2020-05-21;5(10):.
    Species: Human
    Sample Types: Serum
  34. Effects of dietary methionine and cysteine restriction on plasma biomarkers, serum fibroblast growth factor 21, and adipose tissue gene expression in women with overweight or obesity: a double-blind randomized controlled pilot study
    Authors: T Olsen, B Øvrebø, N Haj-Yasein, S Lee, K Svendsen, M Hjorth, NE Bastani, F Norheim, CA Drevon, H Refsum, KJ Vinknes
    J Transl Med, 2020-03-11;18(1):122.
    Species: Human
    Sample Types: Serum
  35. Enhanced GIP Secretion in Obesity Is Associated with Biochemical Alteration and miRNA Contribution to the Development of Liver Steatosis
    Authors: J Góralska, U Ra?ny, A Polus, A Dziewo?ska, A Gruca, A Zdzienicka, A Dembi?ska-, B Solnica, A Micek, M Kapusta, K S?owi?ska-, M Malczewska
    Nutrients, 2020-02-13;12(2):.
    Species: Human
    Sample Types: Plasma
  36. Effects of dapagliflozin on the serum levels of fibroblast growth factor 21 and myokines and muscle mass in Japanese patients with type 2 diabetes: a randomized, controlled trial
    Authors: H Yamakage, M Tanaka, T Inoue, S Odori, T Kusakabe, N Satoh-Asah
    J Diabetes Investig, 2019-12-10;0(0):.
    Species: Human
    Sample Types: Serum
  37. Myokine/Adipokine Response to "Aerobic" Exercise: Is It Just a Matter of Exercise Load?
    Authors: Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia
    Front Physiol, 2019-05-29;10(0):691.
    Species: Human
    Sample Types: Serum
  38. FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics
    Authors: X Li, Y Hong, H He, G Jiang, W You, X Liang, Q Fu, S Han, Q Lian, Y Zhang
    Oxid Med Cell Longev, 2019-04-17;2019(0):4915149.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output
    Authors: J Liu, K Yang, J Yang, W Xiao, Y Le, F Yu, L Gu, S Lang, Q Tian, T Jin, R Wei, T Hong
    EBioMedicine, 2019-03-01;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Myokine Response to High-Intensity Interval vs. Resistance Exercise: An Individual Approach
    Authors: Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia
    Front Physiol, 2018-12-03;9(0):1735.
    Species: Human
    Sample Types: Serum
  41. A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance
    Authors: J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann
    Front Physiol, 2018-11-28;9(0):1711.
    Species: Human
    Sample Types: Serum
  42. Circulating level of fibroblast growth factor 21 is independently associated with the risks of unstable angina pectoris
    Authors: J Cheng, X Su, L Qiao, C Zhai, W Chen
    Biosci. Rep., 2018-09-25;0(0):.
    Species: Human
    Sample Types: Serum
  43. FGF21, a liver hormone that inhibits alcohol intake in mice, increases in human circulation after acute alcohol ingestion and sustained binge drinking at Oktoberfest
    Authors: S Søberg, ES Andersen, NB Dalgaard, I Jarlhelt, NL Hansen, N Hoffmann, T Vilsbøll, A Chenchar, M Jensen, TJ Grevengoed, SAJ Trammell, FK Knop, MP Gillum
    Mol Metab, 2018-03-27;0(0):.
    Species: Human
    Sample Types: Plasma
  44. Epigenetic modulation of Fgf21 in the perinatal mouse liver ameliorates diet-induced obesity in adulthood
    Authors: X Yuan, K Tsujimoto, K Hashimoto, K Kawahori, N Hanzawa, M Hamaguchi, T Seki, M Nawa, T Ehara, Y Kitamura, I Hatada, M Konishi, N Itoh, Y Nakagawa, H Shimano, T Takai-Igar, Y Kamei, Y Ogawa
    Nat Commun, 2018-02-12;9(1):636.
    Species: Mouse
    Sample Types: Serum
  45. Plasma Levels of Myonectin But Not Myostatin or Fibroblast-Derived Growth Factor 21 Are Associated with Insulin Resistance in Adult Humans without Diabetes Mellitus
    Authors: FJK Toloza, JO Mantilla-R, MC Pérez-Mato, ML Ricardo-Si, MC Morales-Al, JA Pinzón-Cor, M Pérez-Mayo, ML Arévalo-Ga, G Tolosa-Gon, CO Mendivil
    Front Endocrinol (Lausanne), 2018-01-31;9(0):5.
    Species: Human
    Sample Types: Plasma
  46. Fibroblast growth factor 21 (FGF21) is robustly induced by ethanol and has a protective role in ethanol associated liver injury
    Authors: BN Desai, G Singhal, M Watanabe, D Stevanovic, T Lundasen, FM Fisher, ML Mather, HG Vardeh, N Douris, AC Adams, IA Nasser, GA FitzGerald, JS Flier, C Skarke, E Maratos-Fl
    Mol Metab, 2017-08-19;6(11):1395-1406.
    Species: Human
    Sample Types: Serum
  47. Age-Associated Loss of OPA1 in Muscle Impacts Muscle Mass, Metabolic Homeostasis, Systemic Inflammation, and Epithelial Senescence
    Authors: C Tezze, V Romanello, MA Desbats, GP Fadini, M Albiero, G Favaro, S Ciciliot, ME Soriano, V Morbidoni, C Cerqua, S Loefler, H Kern, C Franceschi, S Salvioli, M Conte, B Blaauw, S Zampieri, L Salviati, L Scorrano, M Sandri
    Cell Metab., 2017-05-25;0(0):.
    Species: Human
    Sample Types: Plasma
  48. Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases
    Authors: T Matsuhashi, T Sato, SI Kanno, T Suzuki, A Matsuo, Y Oba, M Kikusato, E Ogasawara, T Kudo, K Suzuki, O Ohara, H Shimbo, F Nanto, H Yamaguchi, D Saigusa, Y Mukaiyama, A Watabe, K Kikuchi, H Shima, E Mishima, Y Akiyama, Y Oikawa, HO Hsin-Jung, Y Akiyama, C Suzuki, M Uematsu, M Ogata, N Kumagai, M Toyomizu, A Hozawa, N Mano, Y Owada, S Aiba, T Yanagisawa, Y Tomioka, S Kure, S Ito, K Nakada, KI Hayashi, H Osaka, T Abe
    EBioMedicine, 2017-05-13;0(0):.
    Species: Human
    Sample Types: Serum
  49. The Relationship between Mitochondrial Respiratory Chain Activities in Muscle and Metabolites in Plasma and Urine: A Retrospective Study
    Authors: C Alban, E Fatale, A Joulani, P Ilin, A Saada
    J Clin Med, 2017-03-10;6(3):.
    Species: Human
    Sample Types: Plasma
  50. Circulating FGF21 in humans is potently induced by short term overfeeding of carbohydrates
    Authors: AM Lundsgaard, AM Fritzen, KA Sj?berg, LS Myrmel, L Madsen, JF Wojtaszews, EA Richter, B Kiens
    Mol Metab, 2016-11-16;6(1):22-29.
    Species: Human
    Sample Types: Plasma
  51. Raised FGF-21 and Triglycerides Accompany Increased Energy Intake Driven by Protein Leverage in Lean, Healthy Individuals: A Randomised Trial
    PLoS ONE, 2016-08-18;11(8):e0161003.
    Species: Human
    Sample Types: Plasma
  52. CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
    Authors: Jong-Gil Park
    Sci Rep, 2016-06-15;6(0):27938.
    Species: Mouse
    Sample Types: Plasma
  53. Lack of Day/Night variation in fibroblast growth factor 21 levels in young healthy men.
    Authors: Foo J, Aronis K, Chamberland J, Mantzoros C
    Int J Obes (Lond), 2014-12-26;39(6):945-8.
    Species: Human
    Sample Types: Serum
  54. Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus: association with insulin resistance, adiponectin, and polycystic ovary syndrome history.
    Authors: Wang D, Zhu W, Li J, An C, Wang Z
    PLoS ONE, 2013-11-19;8(11):e81190.
    Species: Human
    Sample Types: Serum
  55. Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine.
    Authors: Besse-Patin, A, Montastier, E, Vinel, C, Castan-Laurell, I, Louche, K, Dray, C, Daviaud, D, Mir, L, Marques, M-A, Thalamas, C, Valet, P, Langin, D, Moro, C, Viguerie, N
    Int J Obes (Lond), 2013-08-27;38(5):707-13.
    Species: Human
    Sample Types: Plasma

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Human FGF-21 Quantikine ELISA Kit
By Anonymous on 11/10/2023
Sample Tested: Plasma
Human FGF-21 Quantikine ELISA Kit DF2100
Human FGF-21 Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Serum and Plasma
Human FGF-21 Quantikine ELISA Kit
By Anonymous on 03/17/2022
Sample Tested: Serum
Human FGF-21 Quantikine ELISA Kit DF2100
Human FGF-21 Quantikine ELISA Kit
By Anonymous on 01/31/2022
Sample Tested: Serum
Human FGF-21 Quantikine ELISA Kit DF2100
Human FGF-21 Quantikine ELISA Kit
By Anonymous on 01/27/2022
Sample Tested: EDTA Plasma
Human FGF-21 Quantikine ELISA Kit DF2100
Human FGF-21 Quantikine ELISA Kit
By Jessica Fernando on 10/23/2017
Sample Tested: Serum