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Human FGF-3 Antibody

Catalog #: MAB1206
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1206
MAB1206-SP
Cell Proliferation Induced by FGF‑3 and Neutralization by Human FGF‑3 Antibody.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -18, -19, -20, -21, -23, rhFGF acidic, rhFGF basic, recombinant mouse (rm) FGF-8b, -8c, or
-15 is observed.
Source
Monoclonal Mouse IgG2A Clone # 254625
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FGF-3
Asp28-Arg212
Accession # P11487
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF‑3 (Catalog # 1206-F3)
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue
Neutralization
Measured by its ability to neutralize FGF‑3-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 2-5 µg/mL in the presence of 0.3 µg/mL Recombinant Human FGF‑3 and 1 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF‑3 and Neutralization by Human FGF‑3 Antibody. View Larger

Cell Proliferation Induced by FGF‑3 and Neutralization by Human FGF‑3 Antibody. Recombinant Human FGF-3 (Catalog # 1206-F3) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-3 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-3 Monoclonal Antibody (Catalog # MAB1206). The ND50 is typically 2-5 µg/mL in the presence of heparin (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-3

Fibroblast Growth Factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

The FGF-3 gene, originally designated int-2, was first identified as a proto-oncogene activated in mouse mammary tumors by proviral integration (2). Amplification of this gene has also been found frequently in human tumors. Human FGF-3 cDNA predicts a 239 aa precursor protein with a 17 aa signal peptide and a 222 aa secreted mature protein with one potential N-linked glycosylation site (1). Human and mouse FGF-3 share 88% aa sequence identity. The Xenopus and mammalian secreted FGF-3 are processed proteolytically at both the N- and C-terminus (5). FGF-3 binds with high-affinity to the IIIb isoforms of FGF R1 and FGF R2. FGF-3 also binds the IIIc isoform of FGF R2, but with lower affinity (6). FGF-3 has been implicated in the induction of inner ear development (7). Studies have suggested that FGF-3 and FGF-8 function synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation (8 - 10). During development, the activities of FGF-3 and FGF-8 are regulated negatively by the sprouty family proteins and by Sef (similar expression to fgf genes), a transmebrane protein that shares intracellular sequence similarities with the IL-17 receptor (10).

References
  1. Brookes, S. et al. (1989) Oncogene 4:429.
  2. Dickson, C. et al. (1989) Prog. Growth Factor Res. 1:123.
  3. Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311. 
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Antoine, M. et al. (2000) Cell Growth Differen. 11:593.
  6. Kohl, R. et al. (2002) J. Biol. Chem. 277:32760.
  7. Represa, J. et al. (1991) Nature 353:561.
  8. Maroon, H. et al. (2002) Development, 129:2099.
  9. Walshe, J. et al. (2002) Current Biol. 12:1117.
  10. Furthauer, M. et al. (2002) Nature Cell Biol. 4:170.
Long Name
Fibroblast Growth Factor 3
Entrez Gene IDs
2248 (Human)
Alternate Names
FGF3; FGF-3; fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog); fibroblast growth factor 3; HBGF-3; Hst; INT-2; mouse; oncogene INT2

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Citations for Human FGF-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A single-step, high-dose selection scheme reveals distinct mechanisms of acquired resistance to oncogenic kinase inhibition in cancer cells
    Authors: KJ Finn, SE Martin, J Settleman
    Cancer Res., 2019-10-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
    Authors: Prignano F, Campolmi P, Bonan P
    Dermatol Ther, 2009-11-01;22(0):S8-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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