Human FGF-5 Antibody Summary
Glu23-Gly268 (Lys238Asn, Pro245Ser)
Accession # Q8NF90
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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FGF‑5 in Human Placenta. FGF-5 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-237-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Cell Proliferation Induced by FGF‑5 and Neutralization by Human FGF‑5 Antibody. Recombinant Human FGF‑5 (Catalog # 237-F5) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑5 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-237-NA). The ND50 is typically 0.2-0.8 µg/mL in the presence of heparin (1 µg/mL).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-5
The FGF family is comprised of at least seven polypeptides that are potent regulators of cell proliferation, differentiation and function. All FGFs have two conserved cysteine residues and share 30-50% sequence homology at the amino acid level. FGF-5 was originally identified as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors. FGF-5 cDNA encodes a 267 amino acid residue protein with a putative 22 amino acid residue signal peptide. The murine homologue of FGF-5 was cloned and found to be 84% homologous to the human protein at the amino acid sequence level. Human and murine FGF-5 exhibit cross species activity.
In vitro, recombinant human FGF-5 is a mitogen for Balb/3T3 fibroblasts and bovine heart endothelial cells. FGF-5 was also reported to be a major muscle-derived survival factor for cultured spinal motoneurons. In vivo, FGF-5 is suggested to play important roles in both embryology and neurobiology. Developmentally, FGF-5 mRNA is initially found in the embryoblast followed by the lateral somatic mesoderm, where it may play a role in angiogenesis, plus the myotomes cranial to the tail region, where it may delay terminal myoblast differentiation during cell migration. FGF-5 continues to impact muscle post-natally where it is believed to function as a target-derived neurotrophic factor of skeletal muscle. In the nervous system, FGF-5 has been most often identified in neurons associated with the limbic system, notably in neurons of the olfactory bulb and pyramidal cells of the hippocampus. Hippocampal FGF-5 is suggested to serve as a neurotrophic and differentiative factor for cholinergic and serotonergic neurons projecting to this region.
Product Datasheets
Citations for Human FGF-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
5
Citations: Showing 1 - 5
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Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
Authors: Haichuan H, Zofia P, Patricia H et al.
Cancer Cell.
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Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210
Authors: Y Tang, S Xiong, P Yu, F Liu, L Cheng
Cell Rep, 2018-07-31;24(5):1355-1362.e3.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
FGF5 is expressed in melanoma and enhances malignancy in vitro and in vivo
Authors: Sara Ghassemi, Katharina Vejdovszky, Emine Sahin, Lukas Ratzinger, Karin Schelch, Thomas Mohr et al.
Oncotarget
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Fibroblast growth factor receptors as therapeutic targets in human melanoma: synergism with BRAF inhibition.
Authors: Metzner T, Bedeir A, Held G, Peter-Vorosmarty B, Ghassemi S, Heinzle C, Spiegl-Kreinecker S, Marian B, Holzmann K, Grasl-Kraupp B, Pirker C, Micksche M, Berger W, Heffeter P, Grusch M
J. Invest. Dermatol., 2011-07-14;131(10):2087-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
FGF5 as an oncogenic factor in human glioblastoma multiforme: autocrine and paracrine activities.
Authors: Allerstorfer S, Sonvilla G, Fischer H, Spiegl-Kreinecker S, Gauglhofer C, Setinek U, Czech T, Marosi C, Buchroithner J, Pichler J, Silye R, Mohr T, Holzmann K, Grasl-Kraupp B, Marian B, Grusch M, Fischer J, Micksche M, Berger W
Oncogene, 2008-03-24;27(30):4180-90.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: IHC, Neutralization, Western Blot
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