Human FGFR2 alpha Antibody

Catalog #: MAB111191 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB111191-100
MAB111191-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGFR2 alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGFR alpha in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1057941

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human FGFR2 alpha
Arg22-Glu377
Accession # P21802.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of FGFR2 alpha in Skin. FGFR2 alpha was detected in immersion fixed paraffin-embedded sections of Skin using Mouse Anti-Human FGFR2 alpha Monoclonal Antibody (Catalog # MAB111191) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2 alpha

Fibroblast growth factor receptor 2 (FGFR2) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing an extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and cytoplasmic split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR2 shares 95% amino acid sequence identity with mouse FGFR2. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR2, alternative splicing of the ECD, specifically the IgIII domain, results in IIIb, or IIIc isoforms (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2, 4). Specifically, FGFR2A (IIIc) binds most FGF ligands but not the FGF10 subfamily, while FGFR2A (IIIc) binds only members of the FGF10 subfamily (5). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (6). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (7). While FGFR2 is widely expressed in many adult human tissues, isoform expression is tissue specific, with IIIb predominantly expressed in epithelial cells, while IIIc is expressed in mesenchymal cells (5). FGFR2 signaling is critical for embryonic development, tissue repair, and regulation of osteoblast function and bone growth (8). Mutations in FGFR2 or misregulation of FGFR2 mediated signaling is found in multiple skeletal dysplasias, with FGFR2A (IIIc) specifically upregulated in several cancers including prostate, breast and pancreatic and is proposed as a novel therapeutic target for colorectal carcinomas (6, 9).

References

Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip Rev Dev Biol. 4:215.
Zhang, X. et al. (2006) J Biol Chem. 281:15694.
Ferguson, H.R. et al. (2021) Signaling. Cells 10:1201.
Holzmann, K. et al. (2012) J Nucleic Acids. 2012:950508.
Wagner, E.J. et al. (2003) RNA 9:1552.
Xie, Y. et al. (2020) Sig Transduct Target Ther 5:181.
Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev Biol. 18:79.
Teven, C.M. et al. (2014) Genes Dis. 1:199.
Matsuda, Y. et al. (2012) Mol Cancer Ther. 11:2010.

Long Name

Fibroblast Growth Factor Receptor 2 alpha

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Alternate Names

BBDS;BEK;BFR-1;CD332;CEK3;CFD1;ECT1;Fibroblast growth factor receptor;Fibroblast growth factor receptor 2;JWS;KGFR;K-SAM;Receptor protein-tyrosine kinase;TK14;TK25; FGF R2a; FGFR2 alpha