Human Fgr Antibody

Catalog #: MAB32071 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB32071
MAB32071-SP

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Human Fgr Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fgr in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 349331

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Fgr in HepG2 Human Cell Line. Fgr was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Fgr Monoclonal Antibody (Catalog # MAB32071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fgr

Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of nonreceptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.

Long Name

Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog

Entrez Gene IDs

2268 (Human); 14191 (Mouse); 79113 (Rat)

Alternate Names

c-fgr; c-src-2 proto-oncogene; c-src2; EC 2.7.10; EC 2.7.10.2; Fgr; FLJ43153; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; MGC75096; p55-c-fgr protein; p55c-fgr; p55-Fgr; p58c-fgr; Proto-oncogene c-Fgr; proto-oncogene tyrosine-protein kinase FGR; Src2; SRC2c-fgr protooncogene; tyrosine-protein kinase Fgr