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Human FHL1 Antibody

Catalog #: MAB5938
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5938
MAB5938-SP
Detection of Human FHL1 by Western Blot.
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Product Details
Citations (3)
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Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FHL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FHL1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 767418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FHL1
Thr93-Asn166
Accession # Q13642
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FHL1 antibody by Western Blot. View Larger

Detection of Human FHL1 by Western Blot. Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FHL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FHL1 antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC). View Larger

FHL1 in SH‑SY5Y Human Cell Line. FHL1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FHL1

FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is an approximately 35 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.

Long Name
Four and a Half LIM Domains Protein 1
Entrez Gene IDs
2273 (Human); 14199 (Mouse); 25177 (Rat)
Alternate Names
bA535K18.1; FHL1; four and a half LIM domains 1; HLH1; HPLH1; KYOT; KYO-T; LIM protein SLIMMER; skeletal muscle LIM-protein1; SLIM1; SLIM1Isl-1 and Mec-3 domains 1; SLIMMER; XMPMA

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Citations for Human FHL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Alphavirus nsP3 organizes into tubular scaffolds essential for infection and the cytoplasmic granule architecture
    Authors: Kril, V;Hons, M;Amadori, C;Zimberger, C;Couture, L;Bouery, Y;Burlaud-Gaillard, J;Karpov, A;Ptchelkine, D;Thienel, AL;Kümmerer, BM;Desfosses, A;Jones, R;Roingeard, P;Meertens, L;Amara, A;Reguera, J;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Single-cell analysis of arthritogenic alphavirus-infected human synovial fibroblasts links low abundance of viral RNA to induction of innate immunity and arthralgia-associated gene expression
    Authors: Fabian Pott, Dylan Postmus, Richard J. P. Brown, Emanuel Wyler, Elena Neumann, Markus Landthaler et al.
    Emerging Microbes & Infections
  3. FHL1 is a major host factor for chikungunya virus infection
    Authors: L Meertens, ML Hafirassou, T Couderc, L Bonnet-Mad, V Kril, BM Kümmerer, A Labeau, A Brugier, E Simon-Lori, J Burlaud-Ga, C Doyen, L Pezzi, T Goupil, S Rafasse, PO Vidalain, A Bertrand-L, L Gueneau, R Juntas-Mor, R Ben Yaou, G Bonne, X de Lamball, M Benkirane, P Roingeard, C Delaugerre, M Lecuit, A Amara
    Nature, 2019-09-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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