Human Fibromodulin/FMOD PE-conjugated Antibody

Name: Human Fibromodulin/FMOD PE-conjugated Antibody
Brand: R&D Systems
SKU: IC5945P

Catalog #: IC5945P Datasheet / COA / SDS

Discontinued Product

IC5945P has been discontinued.

View all Fibromodulin/FMOD products.

Detection of Fibromodulin/FMOD in MG-63 Human Cell Line by Flow Cytometry.

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All Fibromodulin/FMOD Antibodies

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Human Fibromodulin/FMOD PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fibromodulin/FMOD in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Lumican is observed.

Source

Monoclonal Mouse IgG_2B Clone # 549302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
Asp75-Ile376
Accession # NP_002014

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Fibromodulin/FMOD antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

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Detection of Fibromodulin/FMOD in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human Fibromodulin/FMOD PE-conjugated Monoclonal Antibody (Catalog # IC5945P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Fibromodulin/FMOD

Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50‑70 kDa protein contains a sulfated N‑terminal complement C1q binding domain (aa 19‑98) and 12 leucine-rich repeats (aa 75‑376). A 40 kDa form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa sequence identity with mouse and rat FMOD.

Entrez Gene IDs

2331 (Human); 14264 (Mouse); 64507 (Rat)

Alternate Names

Collagen-binding 59 kDa protein; fibromodulin proteoglycan; Fibromodulin; FM; FMOD; Keratan sulfate proteoglycan fibromodulin; SLRR2E; SLRR2EKSPG fibromodulin