Human Ficolin-1 Antibody

Catalog #: AF4209 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4209
AF4209-SP

All Ficolin-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ficolin-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ficolin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) Ficolin-2 is observed and less than 1% cross-reactivity with rhFicolin-3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ficolin-1
Gln28-Ala326
Accession # O00602

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Ficolin‑1 (Catalog # 4209-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ficolin-1

Human Ficolin-1 (fibrinogen/collagen-like), also called M-ficolin, is a member of the ficolin family of secreted pattern recognition proteins in the lectin complement activation pathway (1‑3). Ficolin-1 is expressed by monocytes, neutrophils and type II alveolar epithelial cells (2‑5). It is proposed to be a locally-acting lectin released in a regulated manner (4). Ficolin-1 is not found in plasma, but is detected on the surface of circulating monocytes (4‑6). The 35 kDa, 326 amino acid (aa) human Ficolin-1 contains a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that includes a calcium binding site and one potential N-glycosylation site. Both the collagen and FBG domains mediate trimer formation (7). Like Ficolin-2, larger homo-multimers of Ficolin-1 exist and are likely formed by disulfide bonds at the N-terminus. Sizes corresponding to 12 or 18 subunit oligomers are reported (4, 6, 7). The FBG domain of Ficolin-1 binds microbial ligands that contain acetylated compounds (6). Ligands identified include N-acetyl glucosamine, N-acetyl galactosamine and sialyl-N-acetyllactosamine (4‑7). Like other ficolins, Ficolin-1 associates with, and activates the MBL-associated serine protease (MASP) complex, which activates the complement pathway by cleaving C4, contributing to the innate immune response (4‑6). Mature human Ficolin-1 shares 76%, 61%, 61%, 76% and 81% aa identity with mouse Ficolin-2 (8), and mouse, rat, canine and porcine Ficolin-1, respectively. It shares 84% and 46% aa identity with human Ficolin-2 and Ficolin-3, respectively. The Ficolin-1 gene is polymorphic, showing at least ten single nucleotide polymorphisms in the promoter and coding regions (1).

References

Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
Endo, Y. et al. (1996) Genomics 36:515.
Lu, J. et al. (1996) Biochem. J. 313: 473.
Liu, Y. et al. (2005) J. Immunol. 175:3150.
Teh, C. et al. (2000) Immunology 101:225.
Frederiksen, P. D. et al. (2005) Scand. J. Immunol. 62:462.
Tanio, M. et al. (2007) J. Biol. Chem. 282:3889.
Endo, Y. et al. (2004) Genomics 84:737.

Long Name

Ficolin [Collagen/Fibrinogen Domain Containing] 1

Entrez Gene IDs

2219 (Human); 14133 (Mouse)

Alternate Names

Collagen/fibrinogen domain-containing protein 1; FCN1; Fcna; FCNM; FCNMficolin-A; ficolin (collagen/fibrinogen domain containing) 1; ficolin (collagen/fibrinogen domain-containing) 1; Ficolin1; Ficolin-1; Ficolin-A; ficolin-alpha; M-ficolin