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Human Ficolin-3 Biotinylated Antibody

Catalog #: BAF2367
3 Citations Datasheet / COA / SDS
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BAF2367
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Citations (3)
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Human Ficolin-3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ficolin‑3 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Ficolin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ficolin-3
Lys22-Arg299
Accession # O75636-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Ficolin‑3 (Catalog # 2367-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ficolin-3

Human Ficolin-3 (fibrinogen/collagen-like), also called H-ficolin and, previously, Hakata antigen or thermolabile beta -2 macroglycoprotein, is a member of the ficolin family of secreted pattern recognition proteins that belong to the lectin complement activation pathway (1, 2). Ficolin-3 is expressed by bile duct epithelial cells and hepatocytes, and is released into the bile and circulation, where it averages 18 μg/mL (3, 4). It is also secreted by bronchial and alveolar epithelial cells in the lung (3). Mature human Ficolin-3 shares 46% and 52% amino acid (aa) identity with human Ficolin-1 and Ficolin-2, respectively. Ficolin-3 has only been identified in primates and is likely a pseudogene in other species (5). The 35 kDa, 288 aa human Ficolin-3 (isoform 2) contains a signal sequence, an N-terminal collagen domain and a C‑terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. Isoform 1 contains an additional 11 aa between the collagen and fibrinogen-like domains. The collagen domain mediates trimer formation, and a ~650 kDa, 18 subunit oligomer is formed by disulfide links at the N‑terminus (2, 6, 7). Ficolin-3 binds a limited set of carbohydrates containing mannose, galactose or D-fucose (2, 6). Binding of microbial carbohydrates has been clearly demonstrated only for the PSA antigen of Aerococcus viridans (4, 8, 9). Pathogen recognition initiates an immune response involving the calcium-dependent interaction of Ficolin-3 with the MBL-associated serine protease (MASP) complex. This cleaves C4 to activate the complement pathway (4, 9). In a secondary role, Ficolins 2 and 3 bind apoptotic cells, activating complement cascades that assist in clearance of the cells (10). Circulating antibodies to Ficolin-3 have been identified in systemic lupus erythematosus (7).

References
  1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
  2. Sugimoto, R. et al. (1998) J. Biol. Chem. 273:20721.
  3. Akaiwa, M. et al. (1999) J. Histochem. Cytochem. 47:777.
  4. Krarup, A. et al. (2005) Inf. Immun. 72:1052.
  5. Endo, Y. et al. (2004) Genomics 84:737.
  6. Garlatti, V. et al. (2007) EMBO J. 26:623.
  7. Yae, Y. et al. (1991) Biochim. Biophys. Acta 1078:369.
  8. Tsujimura, M. et al. (2001) Clin. Diag. Lab. Immunol. 8:454.
  9. Matsushita, M. et al. (2002) J. Immunol. 168:3502.
  10. Kuraya, M. et al. (2005) Immunobiology 209:689.
Long Name
Ficolin [Collagen/Fibrinogen Domain Containing] 3
Entrez Gene IDs
8547 (Human)
Alternate Names
FCN3; FCNH; Ficolin3; Ficolin-3; HAKA1; H-Ficolin

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Citations for Human Ficolin-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Circulating Ficolin-2 and Ficolin-3 Form Heterocomplexes
    Authors: Ida Jarlhelt, Katrine Pilely, Jytte Bryde Clausen, Mikkel-Ole Skjoedt, Rafael Bayarri-Olmos, Peter Garred
    The Journal of Immunology
  2. Synergy between ficolin-2 and pentraxin 3 boosts innate immune recognition and complement deposition.
    Authors: Ma YJ, Doni A, Hummelshoj T, Honore C, Bastone A, Mantovani A, Thielens NM, Garred P
    J. Biol. Chem., 2009-07-24;284(41):28263-75.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. Allelic Lineages of the Ficolin Genes (FCNs) Are Passed from Ancestral to Descendant Primates
    Authors: Tina Hummelshøj, Janna Nissen, Lea Munthe-Fog, Claus Koch, Mads Frost Frost Bertelsen, Peter Garred
    PLoS ONE

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