Human FKBP12 Antibody

Catalog #: MAB37771 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB37771-100
MAB37771-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FKBP12 Antibody Summary

Species Reactivity

Human

Specificity

Detects both Human FKBP12 and FKBP12^F36V mutant in direct Elisas

Source

Monoclonal Mouse IgG_2B Clone # 1049713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived human FKBP12^F36V protein
Gly1-Glu108
Accession # P62942

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue

Immunocytochemistry

3-25 µg/mL

Immersion fixed A431 human epidermoid carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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FKBP12 in A431 human cell line. FKBP12 was detected in immersion fixed A431 human epidermoid carcinoma cell line using Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Adherent Cells.

Western Blot

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Detection of Human, Mouse, and Rat FKBP12 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FKBP12 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Specificity of FKBP12 Shown by Western Blot. Western blot shows recombinant human FKBP(F36V), FKBP12, and FKBP 12.6. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FKBP(F36V) and FKBP12 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP12

FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity (2) and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).

Long Name

12 kDa FK506 Binding Protein

Entrez Gene IDs

2280 (Human); 14225 (Mouse); 25639 (Rat)

Alternate Names

12 kDa FKBP; EC 5.2.1.8; FK506 binding protein 1A, 12kDa; FK506-binding protein 1A; FKBP1; FKBP12; FKBP12C; FKBP12FKBP12-Exip3; FKBP-12T-cell, 12-kD; FKBP1a; FKBP-1A; peptidyl-prolyl cis-trans isomerase FKBP1A; PKC12; PPIase FKBP1A; PPIASE; protein kinase C inhibitor 2; rotamase