Human FLRT1 Antibody

Catalog #: AF2794 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2794
AF2794-SP

All FLRT1 Antibodies

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Human FLRT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FLRT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) FLRT3 is observed and less than 1% cross-reactivity with rhFLRT2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FLRT1
Ile21-Pro524
Accession # Q9NZU1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FLRT1 (Catalog # 2794-FL)

Flow Cytometry

2.5 µg/10⁶ cells

HEK293 human embryonic kidney cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLRT1

FLRT1 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 90 kDa type I transmembrane (TM) human FLRT1 is synthesized as a 646 amino acid (aa) precursor with a 20 aa signal sequence, a 504 aa extracellular domain (ECD), a 21 aa TM segment and a 101 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT1 ECD shares 97% aa sequence identity with mouse, rat, and canine, and 95% with bovine FLRT1 ECD. Human FLRT2 and FLRT3 ECDs share 47% and 61% aa identity, respectively, with FLRT1. The fibronectin domain of all three FLRTs can bind FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). Both during development and in adulthood, FLRT1 mRNA is predominantly expressed in brain and kidney (1). FLRT1 is expressed at brain compartmental boundaries in mouse embryos (2). Its expression is distinct from that of FLRT2 and FLRT3 (2).

References

Lacy, S. E. et al. (1999) Genomics 62:417.
Haines, B. P. et al. (2006) Dev. Biol. 297:14.
Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
Ogata, S. et al. (2007) Genes Dev. 21:1817.

Long Name

Fibronectin Leucine Rich Transmembrane Protein 1

Entrez Gene IDs

23769 (Human); 396184 (Mouse)

Alternate Names

fibronectin leucine rich transmembrane protein 1; Fibronectin-like domain-containing leucine-rich transmembrane protein 1; FLRT1; leucine-rich repeat transmembrane protein FLRT1; MGC21624; SPG68