Human Follistatin Antibody Summary
Gly30-Asp329
Accession # P19883
Applications
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Follistatin in Human Pituitary. Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin
Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).
- Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218.
- Thompson, T.B. et al. (2005) Dev. Cell 9:535.
- Sidis, Y. et al. (2005) Endocrinology 146:130.
- Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228.
- Sidis, Y. et al. (2006) Endocrinology 147:3586.
- Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
- Gao, X. et al. (2007) FEBS Lett. 581:5505.
- Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
- Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
- Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.
Product Datasheets
Citations for Human Follistatin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitis
Authors: Sasaki M, Hara T, Wang JX et al.
bioRxiv : the preprint server for biology
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Follistatin impacts Tumor Angiogenesis and Outcome in Thymic Epithelial Tumors
Authors: S Janik, C Bekos, P Hacker, T Raunegger, AI Schiefer, L Müllauer, C Veraar, B Dome, W Klepetko, HJ Ankersmit, B Moser
Sci Rep, 2019-11-22;9(1):17359.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro
Authors: W Wu, XL Wu, YQ Ji, Z Gao
Mol Med Rep, 2017-05-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
BMP-driven NRF2 activation in esophageal basal cell differentiation and eosinophilic esophagitis.
Authors: Jiang, Ming, Ku, Wei-Yao, Zhou, Zhongren, Dellon, Evan S, Falk, Gary W, Nakagawa, Hiroshi, Wang, Mei-Lun, Liu, Kuancan, Wang, Jun, Katzka, David A, Peters, Jeffrey, Lan, Xiaopeng, Que, Jianwen
J Clin Invest, 2015-03-16;125(4):1557-68.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Nucleolar follistatin promotes cancer cell survival under glucose-deprived conditions through inhibiting cellular rRNA synthesis.
Authors: Gao X, Wei S, Lai K
J. Biol. Chem., 2010-09-15;285(47):36857-64.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells.
Authors: Kobayashi T, Iwasaki T, Amagai M
J. Invest. Dermatol., 2010-04-01;130(8):1988-95.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-Fr -
Thapsigargin resistance in human prostate cancer cells.
Authors: O'Neill JP, Velalar CN, Lee DI, Zhang B, Nakanishi T, Tang Y, Selaru F, Ross D, Meltzer SJ, Hussain A
Cancer, 2006-08-01;107(3):649-59.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The activin-follistatin system and in vitro early follicle development in goats.
Authors: Silva JR, Tharasanit T, Taverne MA, van der Weijden GC, Santos RR, Figueiredo JR, van den Hurk R
J. Endocrinol., 2006-04-01;189(1):113-25.
Species: Goat
Sample Types: Whole Tissue
Applications: IHC-P -
Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts.
Authors: Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J
Arthritis Rheum., 2004-08-01;50(8):2521-30.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
J. Bone Miner. Res., 2004-05-03;19(8):1302-7.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
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