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Human FoxC2 Antibody

Catalog #: MAB5044
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5044
MAB5044-SP
Detection of Human FoxC2 by Western Blot.
2 Images
Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxC2 in direct ELISAs and Western blots. Approximately 25% cross-reactivity with recombinant human (rh) FoxA1 (aa 253‑472) is observed in Western blots but not in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with rhFoxB1 (aa 191‑324), D3 (aa 1-140), F1 (aa 129-354), J1 (aa 306-421), K1 (aa 493-670), M1 (aa 642-763), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179‑279), or P4 (aa 229-410) is observed.
Source
Monoclonal Mouse IgG2A Clone # 665819
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FoxC2
Gly415-Tyr501
Accession # Q99958
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FoxC2 antibody by Western Blot. View Larger

Detection of Human FoxC2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, T47D human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FoxC2 antibody in MDA-MB-453 Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxC2 in MDA‑MB‑453 Human Cell Line. FoxC2 was detected in immersion fixed MDA-MB-453 human breast cancer cell line using Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxC2

FoxC2 belongs to a large family of nuclear transcription factor proteins sharing a common forkhead/winged helix DNA binding domain. FoxC2 is implicated in epithelial to mesenchymal transition, human lymphedemia-distichiasis syndrome, and tumor metastasis. Experiments in mice indicate that FoxC2 controls adipocyte morphogenesis and null mice show defects in axial and cranial skeletogenesis, as well. In addition the transcriptional activity of FoxC2 influences expression of cytokine receptors such as CXCR4.

Long Name
Forkhead Box C2
Entrez Gene IDs
2303 (Human); 14234 (Mouse)
Alternate Names
Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14

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Citation for Human FoxC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hemodynamic regulation of perivalvular endothelial gene expression prevents deep venous thrombosis
    Authors: JD Welsh, MH Hoofnagle, S Bamezai, M Oxendine, L Lim, JD Hall, J Yang, S Schultz, JD Engel, T Kume, G Oliver, JM Jimenez, ML Kahn
    J. Clin. Invest., 2019-12-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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