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Human FoxC2 Antibody

Catalog #: AF5044
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5044
AF5044-SP
Detection of Human FoxC2 by Western Blot.
3 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxC2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxC2
Gly415-Tyr501
Accession # Q99958
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FoxC2 antibody by Western Blot. View Larger

Detection of Human FoxC2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for FoxC2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FoxC2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxC2 in HeLa Human Cell Line. FoxC2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human FoxC2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human FoxC2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxC2 at approximately 73 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxC2

FoxC2 belongs to a large family of nuclear transcription factor proteins sharing a common forkhead/winged helix DNA binding domain. FoxC2 is implicated in epithelial to mesenchymal transition, human lymphedemia-distichiasis syndrome, and tumor metastasis. Experiments in mice indicate that FoxC2 controls adipocyte morphogenesis and null mice show defects in axial and cranial skeletogenesis, as well. In addition the transcriptional activity of FoxC2 influences expression of cytokine receptors such as CXCR4.

Long Name
Forkhead Box C2
Entrez Gene IDs
2303 (Human); 14234 (Mouse)
Alternate Names
Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14

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Citations for Human FoxC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Piezo1 incorporates mechanical force signals to genetic program that governs lymphatic valve development and maintenance
    Authors: D Choi, E Park, E Jung, B Cha, S Lee, J Yu, PM Kim, S Lee, YJ Hong, CJ Koh, CW Cho, Y Wu, NL Jeon, AK Wong, L Shin, SR Kumar, I Bermejo-Mo, RS Srinivasan, IT Cho, YK Hong
    JCI Insight, 2019-03-07;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Human venous valve disease caused by mutations in FOXC2 and GJC2
    Authors: Oliver Lyons, Prakash Saha, Christopher Seet, Adam Kuchta, Andrew Arnold, Steven Grover et al.
    Journal of Experimental Medicine
  3. Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2
    Authors: Johanna Liebl, Siwei Zhang, Markus Moser, Yan Agalarov, Cansaran Saygili Demir, Bianca Hager et al.
    Nature Communications
  4. Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
    Authors: Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S
    Clin Cancer Res, 2014-12-16;21(4):899-906.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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