Human FoxP1 APC-conjugated Antibody

Catalog #: IC45341A Datasheet / COA / SDS

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IC45341A

Detection of FoxP1 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human FoxP1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxP1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 837016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FoxP1
Lys548-Glu677
Accession # Q9H334

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of FoxP1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

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Detection of FoxP1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FoxP1 APC-conjugated Monoclonal Antibody (Catalog # IC45341A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: FoxP1

Forkhead Box P1 (FOXP1) is a member of the FOX family of transcription factors. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 contains both a DNA binding domain as well as protein-protein interaction domains. FoxP1 can homo or heterodimerize with FoxP2 and FoxP4, with dimerization necessary for DNA binding. FoxP1 shows both oncogenic and tumor suppressive characteristics. Overexpression in lymphomas leads to poor prognosis, but loss of FoxP1 in breast cancer also implicates a poor prognosis. Human isoforms of 489 to 677 amino acids contain alternate sequences within the first 60 amino acids and/or deletion of amino acids.

Long Name

Forkhead Box P1

Entrez Gene IDs

27086 (Human); 108655 (Mouse); 297480 (Rat)

Alternate Names

FLJ23741; fork head-related protein like B; forkhead box P1; forkhead box protein P1; FoxP1; glutamine-rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF1; QRF112CC4