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Human FPRL1/FPR2 Antibody

Catalog #: MAB3479
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3479
MAB3479-SP
Detection of FPRL1/FPR2 in Human Blood Monocytes by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FPRL1/FPR2 Antibody Summary

Species Reactivity
Human
Specificity
Stains FPRL1 transfectants but not irrelevant transfectants. It also stains monocytes and granulocytes.
Source
Monoclonal Mouse IgG2B Clone # 304405
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FPRL1
Met1-Met351
Accession # P25090
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FPRL1/FPR2 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of FPRL1/FPR2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human FPRL1/ FPR2 Monoclonal Antibody (Catalog # MAB3479, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRL1/FPR2

FPRL1 is a 7TM protein expressed on mononuclear phagocytes and microglial cells. It interacts with formyl peptides to attract phagocytes to sites of infection and promote inflammatory reactions. FPRL1 also interacts with amyloid beta peptides and has been implicated in phagocyte attraction to sites of amyloid plaques in Alzheimer’s disease.

Long Name
Formyl Peptide Receptor-like 1
Entrez Gene IDs
2358 (Human); 14289 (Mouse)
Alternate Names
ALXR; FMLP-R-I; FMLP-R-II; FMLPX; formyl peptide receptor 2; Formyl peptide receptor-like 1RFP; FPR2; FPR2A; FPRH1FMLP-related receptor I; FPRL1; FPRL1LXA4 receptor; HM63; HM63FPRH2; lipoxin A4 receptor (formyl peptide receptor related); Lipoxin A4 receptor; LXA4 Receptor; LXA4RFMLP-R-I; N-formyl peptide receptor 2; RFP

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Citations for Human FPRL1/FPR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Annexin A1, FPR2/ALX, and inflammatory cytokine expression in peritoneal endometriosis
    Authors: LK Volpato, VV Horewicz, F Bobinski, DF Martins, AP Piovezan
    J. Reprod. Immunol., 2018-08-03;129(0):30-35.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Intracellular formyl peptide receptor regulates na�ve CD4 T cell migration
    Authors: HY Lee, YS Jeong, M Lee, HS Kweon, YH Huh, JS Park, JE Hwang, K Kim, YS Bae
    Biochem. Biophys. Res. Commun., 2018-02-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2014-09-29;34(29):3826-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Aspirin-triggered lipoxin and resolvin E1 modulate vascular smooth muscle phenotype and correlate with peripheral atherosclerosis.
    Authors: Ho KJ, Spite M, Owens CD, Lancero H, Kroemer AH, Pande R, Creager MA, Serhan CN, Conte MS
    Am. J. Pathol., 2010-08-13;177(4):2116-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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