Human FRS2 Antibody

Catalog #: MAB40691 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB40691
MAB40691-SP

Detection of FRS2 in A172 Human Cell Line by Flow Cytometry.

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All FRS2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FRS2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FRS2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 462902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FRS2
Asn121-Asn449
Accession # Q8WU20

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of FRS2 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of FRS2 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human FRS2 Monoclonal Antibody (Catalog # MAB40691, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGFR substrate 2; also SNT and FSR2-alpha ) is a 70‑90 kDa member of the FSR family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors, and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids (aa) in length. It contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196‑471). One splice form shows a deletion of the C-terminal four amino acids. Over aa 121‑449, human FRS2 is 99% and 94% aa identical to dog and mouse FRS2, respectively.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Alternate Names

FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1