Human FSH beta Antibody

Catalog #: MAB43101 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43101-100
MAB43101-SP

Detection of Human FSH beta by Western Blot.

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Human FSH beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human FSH beta in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 961416

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human FSH beta
Asn19-Glu129
Accession # P01225

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FSH beta by Western Blot. Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FSH beta Monoclonal Antibody (Catalog # MAB43101) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for FSH beta at approximately 18-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSH beta

Follicle-stimulating hormone (FSH; also follitropin) is a 33 kDa heterodimer belonging to the glycoprotein hormones subunit beta family (1). The heterodimer is composed of two glycoproteins linked noncovalently: a common alpha subunit, which is also a component of luteinizing hormone, thyroid stimulating hormone, and chorionic gonadotropin; and a unique beta subunit that confers the protein’s specific biological action and is responsible for the interaction with FSH-receptor. The alpha subunit is 20 kDa and is synthesized as a 116 amino acid (aa) precursor that contains a 24 aa signal sequence and a 92 aa mature chain, which contains two potential sites of N-linked glycosylation (1-2). The beta subunit is 23 kDa and is synthesized as a 129 aa precursor with an 18 aa signal sequence and a 111 aa mature chain, which also contains two potential sites of N-linked glycosylation (1). Human FSH beta shares approximately 90% aa sequence identity with mouse and rat FSH beta. FSH is secreted by gonadotropes of the anterior pituitary gland. Its release is controlled by pulses of gonadotropin-releasing hormone (GnRH), and those pulses, in turn, are subject to the estrogen feedback from the gonads. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. It promotes follicle maturation and spermatogenesis through interactions with FSH-receptor.

References

Van de Wiel, D.F. et al. (1998) J. Mol. Endocrinol. 20:83.
Rathnam, P. and B.B. Saxena (1975) J. Biol. Chem. 250:6735.

Long Name

Follicle Stimulating Hormone, beta

Entrez Gene IDs

2488 (Human); 14308 (Mouse); 25447 (Rat)

Alternate Names

follicle stimulating hormone, beta polypeptide; Follicle-stimulating hormone beta subunit; Follitropin beta chain; follitropin subunit beta; Follitropin; follitropin, beta chain; FSH beta; FSHB; FSH-B; FSH-beta