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Human Galanin R2/GALR2 Antibody

Catalog #: MAB6544 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6544
MAB6544-SP
Detection of Galanin R2/GALR2 in HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP by Flow Cytometry.
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Human Galanin R2/GALR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GAL-R2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 616520
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Galanin R2
Accession # O43603
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Galanin R2/GALR2 antibody in HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP antibody by Flow Cytometry. View Larger

Detection of Galanin R2/GALR2 in HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Galanin R2/GALR2 and eGFP was stained with either (A) Mouse Anti-Human Galanin R2/GALR2 Monoclonal Antibody (Catalog # MAB6544) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry Galanin R2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Galanin R2 in Human Brain. Galanin R2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human Galanin R2 Monoclonal Antibody (Catalog # MAB6544) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells of blood vessels. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galanin R2/GALR2

GALR2 (Galanin Receptor 2) is one of three 54-60 kDa G-protein coupled multipass transmembrane receptors for the Galanin neuropeptide. Galanin is co-expressed with and modulates noradrenaline and serotonin systems. GALR2 is thought to modulate growth and apoptosis. Unlike GALR and GALR3, it is thought to be anti-depressive. GALR2 mRNA is found mainly in the central nervous system (except for cerebral cortex), but also in intestine, heart, kidney, liver and many cancers. The combined extracellular domains of human GALR2 share 82% and 86% amino acid identity with mouse and rat GALR2, respectively.

Long Name
Galanin Receptor Type 2
Entrez Gene IDs
8811 (Human); 29234 (Rat)
Alternate Names
GAL2R; GAL2-R; Galanin R2; galanin receptor 2; galanin receptor type 2; GalaninR2; GALNR2; GALNR2MGC125984; GALR2; GALR-2; MGC125983

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