Human Galectin-1 Biotinylated Antibody Summary
Ala2-Asp135
Accession # P09382
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Galectin‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1152) followed by Streptavidin-HRP. A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-1
The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).
Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3 and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity (1-4).
- Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
- Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
- Hughes, R.C. (2001) Biochimie 83:667.
- R&D Systems’ Cytokine Bulletin; Summer 2002.
Product Datasheets
Citations for Human Galectin-1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates
Authors: HM Hsu, YY Yang, YH Huang, CH Chu, TJ Tu, YT Wu, CJ Chiang, SB Yang, DK Hsu, FT Liu, JH Tai
PloS Neglected Tropical Diseases, 2023-01-03;17(1):e0011016.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay
Authors: Tanja Freichel, Viktoria Heine, Dominic Laaf, Eleanor E. Mackintosh, Sophia Sarafova, Lothar Elling et al.
Macromolecular Bioscience
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Induction of galectin-1 by TLR-dependent PI3K activation enhances epithelial-mesenchymal transition of metastatic ovarian cancer cells
Authors: Ga Bin Park, Yoon Hee Chung, Daejin Kim
Oncology Reports
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Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation
Authors: MN Andersen, M Ludvigsen, N Abildgaard, I Petruskevi, R Hjortebjer, M Bjerre, B Honoré, HJ Møller, NF Andersen
Onco Targets Ther, 2017-04-04;10(0):1977-1982.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection) -
Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins.
Authors: Sioud M, Mobergslien A, Boudabous A, Floisand Y
Int. J. Oncol., 2010-12-09;38(2):385-90.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
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