Human Galectin-14 Antibody

Catalog #: MAB5744
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5744
MAB5744-SP

Detection of Human Galectin‑14 by Western Blot.

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All Galectin-14 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-14 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Galectin-14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, -10, or recombinant mouse Galectin-9 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 511124

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Galectin-14
Met1-Asp139
Accession # Q8TCE9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Galectin‑14 by Western Blot. Western blot shows lysates of human eosinophil. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-14 Monoclonal Antibody (Catalog # MAB5744) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin-14 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-14

Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation.

Entrez Gene IDs

56891 (Human)

Alternate Names

Charcot-Leyden crystal protein 2; CLC2; Gal-14; Galectin14; Galectin-14; lectin, galactoside-binding, soluble, 14; LGALS14; MGC22235; Placental Protein 13-Like Protein; PPL13

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Product Datasheet

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Related Research Areas

Citation for Human Galectin-14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1