Human Galectin-2 Antibody

Catalog #: AF1153 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1153
AF1153-SP

All Galectin-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Galectin-2 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant human (rh) Galectin-1, rhGalectin-4, rhGalectin-7, and rhGalectin-8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Galectin‑2 (R&D Systems, Catalog # 1153-GA)
Met1-Glu132
Accession # P05162

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Galectin‑2 (Catalog # 1153-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-2

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-2 is a monomeric or homodimeric prototype galectin that is expressed in hepatoma, stomach epithelial cells and in colorectal and neural tumors. The functions of Galectin-2 has not been reported. Human and mouse Galectin-2 share approximately 65% amino acid sequence similarity (1-4).

References

Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
Hughes, R.C. (2001) Biochimie 83:667.
R&D Systems Cytokine Bulletin; Summer 2002.

Entrez Gene IDs

3957 (Human); 107753 (Mouse); 171134 (Rat)

Alternate Names

Beta-galactoside-binding lectin L-14-II; GAL2; Gal-2; galectin 2; Galectin2; Galectin-2; HL14gal-2; Lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; LGALS2; MGC75071; S-Lac lectin 2