Human Galectin-7 Antibody

Name: Human Galectin-7 Antibody
Brand: R&D Systems
SKU: MAB1339
Rating: 5 (1 reviews)

Catalog #: MAB1339
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1339
MAB1339-SP

All Galectin-7 Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Galectin-7 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) Galectin‑7 and no cross-reactivity with rhGalectin-1, -2, -3, -4, -8, rmGalectin-1, -3, or -4.

Source

Monoclonal Mouse IgG_2B Clone # 198614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Galectin‑7
Ser2-Phe136
Accession # P47929

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Galectin‑7 (Catalog # 1339-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Human Galectin-7 is a prototype monomeric galectin. It is specifically expressed in stratified epithelia, notably in epidermis, but is barely detectable in epidermal tumors and significantly down regulated or absent from squamous carconima cell lines. The Galectin-7 gene is induced by tumor suppressor protein p53 transcriptional activity following genotoxic events. A pro-apoptotic protein, Galectin-7 functions intracellularly upstream of JNK activation and cytochrome-c release. This protein has been shown to increase the susceptibility of keratinocytes to UVB induced apoptosis, an essential processss in the maintenance of epidermal homeostasis. Cell lines transfected with the Galectin-7 gene localized the protein in the nucleus and intracellularly. Human and mouse Galectin-7 share 79% amino acid homology (4-6).

References

Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
Hughes, R.C. (2002) Biochimie 83:667.
R&D Systems Cytokine Bulletin; Summer 2002.
Bernerd, F. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11329.
Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.

Entrez Gene IDs

3963 (Human); 16858 (Mouse)

Alternate Names

GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Galectin-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Ultraviolet irradiation may generate plane xanthomas on mycosis fungoides.
Authors: Fujimoto, N, Mieno, H, Hosokawa, R, Fujimoto, E, Tajima, S
Br J Dermatol, 2012-09-27;168(1):218-20.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
CSF proteomic fingerprints for HIV-associated cognitive impairment.
Authors: Laspiur JP, Anderson ER, Ciborowski P, Wojna V, Rozek W, Duan F, Mayo R, Rodriguez E, Plaud-Valentin M, Rodriguez-Orengo J, Gendelman HE, Melendez LM
J. Neuroimmunol., 2007-10-22;192(1):157-70.
Species: Human
Sample Types: CSF
Applications: Western Blot