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Human Galectin-8 Antibody

Catalog #: AF1305
30 Citations 5 Reviews Datasheet / COA / SDS
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AF1305
AF1305-SP
Detection of Human Galectin‑8 by Western Blot.
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Product Details
Citations (30)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Galectin-8
Met1-Trp317
Accession # O00214
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Galectin‑8 antibody by Western Blot. View Larger

Detection of Human Galectin‑8 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-8 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Galectin-8 antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

Galectin‑8 in Human Lung. Galectin-8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Galectin-8 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Galectin‑8 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-8

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

References
  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems’ Cytokine Bulletin, Summer, 2002.
  5. Bidon, N. et al. (2001) Gene 274:253.
  6. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
  7. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.
Entrez Gene IDs
3964 (Human); 56048 (Mouse)
Alternate Names
GAL8; Gal-8; galectin 8; Galectin8; Galectin-8; galectin-8g; lectin, galactoside-binding, soluble, 8; LGALS8; PCTA1; PCTA-1Po66 carbohydrate-binding protein; Po66 carbohydrate binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1

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Citations for Human Galectin-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage
    Authors: Koerver L, Papadopoulos C, Liu B et al.
    EMBO Rep.
  2. Heterogeneity in pneumolysin expression governs the fate of Streptococcus pneumoniae during blood-brain barrier trafficking
    Authors: MV Surve, S Bhutda, A Datey, A Anil, S Rawat, A Pushpakara, D Singh, KS Kim, D Chakravort, A Banerjee
    PLoS Pathog., 2018-07-16;14(7):e1007168.
  3. Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms
    Authors: MP Wandel, BH Kim, ES Park, KB Boyle, K Nayak, B Lagrange, A Herod, T Henry, M Zilbauer, J Rohde, JD MacMicking, F Randow
    Nat. Immunol., 2020-06-15;0(0):.
  4. Streptolysin O and its Co-Toxin NAD-glycohydrolase Protect Group A Streptococcus from Xenophagic Killing.
    Authors: O'Seaghdha M, Wessels MR.
    PLoS Pathog
  5. The Cargo Receptor NDP52 Initiates Selective Autophagy by Recruiting the ULK Complex to Cytosol-Invading Bacteria.
    Authors: Ravenhill BJ, Boyle KB, von Muhlinen N et al.
    Mol Cell.
  6. Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation
    Authors: Bass, AR;Egan, MS;Alexander-Floyd, J;Lopes Fischer, N;Doerner, J;Shin, S;
    mBio
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  7. TECPR1 conjugates LC3 to damaged endomembranes upon detection of sphingomyelin exposure
    Authors: Keith B Boyle, Cara J Ellison, Paul R Elliott, Martina Schuschnig, Krista Grimes, Marc S Dionne et al.
    The EMBO Journal
  8. mTOR-regulated mitochondrial metabolism limits mycobacterium-induced cytotoxicity
    Authors: Antonio J. Pagán, Lauren J. Lee, Joy Edwards-Hicks, Cecilia B. Moens, David M. Tobin, Elisabeth M. Busch-Nentwich et al.
    Cell
  9. TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escape
    Authors: Noémie Pied, Coralie F. Daussy, Zoé Denis, Jessica Ragues, Muriel Faure, Richard Iggo et al.
    PLOS Pathogens
  10. The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulence
    Authors: MM Osman, JK Shanahan, F Chu, KK Takaki, ML Pinckert, AJ Pagán, R Brosch, WH Conrad, L Ramakrishn
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-10;119(11):e2122161119.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome
    Authors: I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall
    Cell Reports, 2021-10-09;37(4):109892.
    Species: Human, N/A
    Sample Types: Cell Lysates, Recombinant Protein
    Applications: Western Blot, ELISA Detection
  12. Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy
    Authors: Vinay V Eapen, Sharan Swarup, Melissa J Hoyer, Joao A Paulo, J Wade Harper
    eLife
  13. Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection
    Authors: EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow
    Nature, 2021-05-19;0(0):.
    Species: Complex Species Category
    Sample Types: Whole Cells
    Applications: ICC
  14. Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle
    Authors: DC Badgujar, A Anil, AE Green, MV Surve, S Madhavan, A Beckett, IA Prior, BK Godsora, SB Patil, PK More, SG Sarkar, A Mitchell, R Banerjee, PS Phale, TJ Mitchell, DR Neill, P Bhaumik, A Banerjee
    PloS Pathogens, 2020-11-20;16(11):e1009016.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. LRRK 2 activation controls the repair of damaged endomembranes in macrophages
    Authors: Susanne Herbst, Philip Campbell, John Harvey, Elliott M Bernard, Venizelos Papayannopoulos, Nicholas W Wood et al.
    The EMBO Journal
  16. Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death
    Authors: D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel
    Cell Rep, 2020-08-11;32(6):108008.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  17. Mycobacterium marinum phthiocerol dimycocerosates enhance macrophage phagosomal permeabilization and membrane damage
    Authors: MM Osman, AJ Pagán, JK Shanahan, L Ramakrishn
    PLoS ONE, 2020-07-23;15(7):e0233252.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Transbilayer Movement of Sphingomyelin Precedes Catastrophic Breakage of Enterobacteria-Containing Vacuoles.
    Authors: Cara E, Wanda K, Keith B et al.
    Curr Biol.
  19. Human trophoblast requires galectin-3 for cell migration and invasion
    Authors: Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac
    Sci Rep, 2019-02-14;9(1):2136.
    Species: Human
    Sample Types: Basement Membrane Extract
    Applications: ELISA Development (Detection)
  20. Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells
    Authors: TR Lerner, CJ Queval, A Fearns, U Repnik, G Griffiths, MG Gutierrez
    BMC Biol., 2018-01-04;16(1):1.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Galectin-8-mediated selective autophagy protects against seeded tau aggregation.
    Authors: Falcon B, Noad J, McMahon H, Randow F, Goedert M
    J Biol Chem, 2017-12-27;293(7):2438-2451.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8
    Authors: Michal P. Wandel, Claudio Pathe, Emma I. Werner, Cara J. Ellison, Keith B. Boyle, Alexander von der Malsburg et al.
    Cell Host & Microbe
  23. A Rab20-Dependent Membrane Trafficking Pathway Controls M.�tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity
    Authors: L Schnettger, A Rodgers, U Repnik, RP Lai, G Pei, M Verdoes, RJ Wilkinson, DB Young, MG Gutierrez
    Cell Host Microbe, 2017-05-10;21(5):619-628.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  24. K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells
    PLoS Pathog, 2016-11-22;12(11):e1006027.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
    Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
    Nature, 2014-04-16;509(7500):366-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  26. Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling.
    Authors: Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N
    PLoS ONE, 2012-09-04;7(9):e44400.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  27. Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
    Authors: Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
    FASEB J., 2010-09-27;25(1):242-54.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  28. Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
    Authors: Cueni LN, Detmar M
    Exp. Cell Res., 2009-03-04;315(10):1715-23.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  29. The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
    Authors: Thijssen VL, Hulsmans S, Griffioen AW
    Am. J. Pathol., 2008-01-17;172(2):545-53.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC-P, Western Blot
  30. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses
    Authors: Rivera-Serrano EE, Gonzalez-Lopez O, Das A, Lemon SM.
    Elife

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Reviews for Human Galectin-8 Antibody

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Human Galectin-8 Antibody
By Anonymous on 08/20/2018
Application: WB Sample Tested: Huh-7 human hepatoma cell line Species: Human
Western Blot Human Galectin-8 Antibody AF1305
Human Galectin‑8 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Human recombinant galectin-8 Species: Human

The antibody can detect the higher oligomeric form of galectin-8 here. The 36 kDa version was not detectable due to increasing oligomerization of the protein.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Loading buffer
Dilution: 1:1000

Western Blot Human Galectin‑8 Antibody AF1305
Human Galectin‑8 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

At 1:2000 dilution there was very specific binding and the detection was limited to crypts as observed here.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1500 to 1:2000

Immunohistochemistry Human Galectin‑8 Antibody AF1305
Human Galectin‑8 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human cells Species: Human

This experiment, the cells were stained for galectin-8 antibody and then red color secondary was used to detect it.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000

Immunocytochemistry/Immunofluorescence Human Galectin‑8 Antibody AF1305
Human Galectin‑8 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cancer Cells Species: Human

The antibody is good for IFC also.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000

Immunocytochemistry/Immunofluorescence Human Galectin‑8 Antibody AF1305