Human GASP-1/WFIKKN2 Antibody

Catalog #: MAB20701 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB20701
MAB20701-SP

Detection of GASP-1/WFIKKN2 in Human Brain Cortex.

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Human GASP-1/WFIKKN2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human GASP-2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 288518

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
Leu35-His576
Accession # Q8TEU8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Brain Cortex

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of GASP-1/WFIKKN2 in Human Brain Cortex. GASP-1/WFIKKN2 was detected in immersion fixed paraffin-embedded sections of Human Brain Cortex using Mouse Anti-Human GASP-1/WFIKKN2 Monoclonal Antibody (Catalog # MAB20701) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GASP-1/WFIKKN2

Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP‑1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP‑1 is homologous to human WFIKKNRP and mouse GASP‑2 to human WFIKKN. Human GASP‑1 is the same protein as WFIKKNRP.

WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP‑1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP‑1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP‑2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP‑1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP‑1 inhibits myostatin and the highly related protein, GDF‑11, but not Activin or TGF‑ beta in vitro (1). In addition, GASP‑1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP‑1 is 90% and 55% identical to mouse GASP‑1 and human GASP‑2, respectively.

References

Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
Trexler, M. et al. (2002) Biol. Chem. 383:223.

Long Name

Growth and Differentiation Factor-associated Serum Protein-1

Entrez Gene IDs

124857 (Human); 278507 (Mouse); 287631 (Rat)

Alternate Names

GASP1; GASP-1; Growth and differentiation factor-associated serum protein 1; hGASP-1; WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein; WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2; WAP, FS, Ig, two KU and NTR module related protein; WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2; WFIKKN2; WFIKKN-related protein; WFIKKNRP; WFIKKNRPmultivalent protease inhibitor protein