Human GASP-1/WFIKKN2 Antibody

Catalog #: AF2070
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2070
AF2070-SP

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GASP-1/WFIKKN2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human GASP-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
Leu35-His576
Accession # Q8TEU8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GASP‑1/WFIKKN2 (Catalog # 2070-GS)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human GASP‑1/WFIKKN2 (Catalog # 2070-GS), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human GASP-1 (1.5 µg/mL, Catalog # 2070-GS) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (5 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 18.9 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GASP-1/WFIKKN2

Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP.

WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.

References

Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
Trexler, M. et al. (2002) Biol. Chem. 383:223.

Long Name

Growth and Differentiation Factor-associated Serum Protein-1

Entrez Gene IDs

124857 (Human); 278507 (Mouse); 287631 (Rat)

Alternate Names

GASP1; GASP-1; Growth and differentiation factor-associated serum protein 1; hGASP-1; WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein; WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2; WAP, FS, Ig, two KU and NTR module related protein; WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2; WFIKKN2; WFIKKN-related protein; WFIKKNRP; WFIKKNRPmultivalent protease inhibitor protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GASP-1/WFIKKN2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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