Human GASP-1/WFIKKN2 Biotinylated Antibody Summary
Leu35-His576
Accession # Q8TEU8
Applications
Human GASP-1/WFIKKN2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GASP-1/WFIKKN2
Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP.
WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.
- Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
- Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
- Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
- Trexler, M. et al. (2002) Biol. Chem. 383:223.
Product Datasheets
Citations for Human GASP-1/WFIKKN2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease
Authors: Marissa J Schafer
Cell Metab, 2016-06-14;23(6):1207-15.
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Myostatin as a mediator of sarcopenia versus homeostatic regulator of muscle mass: insights using a new mass spectrometry-based assay
Authors: H. Robert Bergen, Joshua N. Farr, Patrick M. Vanderboom, Elizabeth J. Atkinson, Thomas A. White, Ravinder J. Singh et al.
Skeletal Muscle
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