Human GATA-1 PE-conjugated Antibody Summary
Met1-Ser413
Accession # P15976
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of GATA‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Rat Anti-Human GATA-1 PE-conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: GATA-1
GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).
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Intracellular detection of GATA-1 in MCF-7 cells using the PE-conjugated Human GATA-1 antibody (#IC1779P, orange) at 10 uL/10^6 cells, or Isotype Control antibody (black). Cells were fixed with 2% PFA for 10 min and permeabilized with 0.2% Tween-20 for 20 min prior to staining. Staining buffer: 0.2% Tween-20.
