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Human GATA-2 Fluorescein-conjugated Antibody

Catalog #: IC2046F
1 Citation Datasheet / COA / SDS

Discontinued Product

IC2046F has been discontinued.
View all GATA-2 products.
Detection of GATA‑2 in TF‑1 Human Cell Line by Flow Cytometry.
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Human GATA-2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-2. In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with rhGATA-1, rhGATA-5 and rhGATA-6.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of GATA-2 antibody in TF-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GATA‑2 in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Goat Anti-Human GATA-2 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2046F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GATA-2

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA-4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).

References
  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.
Entrez Gene IDs
2624 (Human)
Alternate Names
endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA2; GATA-2; GATA-binding protein 2NFE1B; MGC2306

Product Datasheets

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Citation for Human GATA-2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Leukemia-initiating HSCs in chronic lymphocytic leukemia reveal clonal leukemogenesis and differential drug sensitivity
    Authors: CL Chiang, EY Hu, L Chang, J Labanowska, K Zapolnik, X Mo, J Shi, TJ Doong, A Lozanski, PS Yan, R Bundschuh, LA Walker, D Gallego-Pe, W Lu, M Long, S Kim, NA Heerema, G Lozanski, JA Woyach, JC Byrd, LJ Lee, N Muthusamy
    Oncogene, 2022-07-19;40(3):111115.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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