Human GATA-3 APC-conjugated Antibody Summary
Pro135-Ser258
Accession # P23771
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of GATA‑3 in Human PBMCs by Flow Cytometry. Th2-stimulated human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N) and either (A) Mouse Anti-Human GATA-3 APC-conjugated Monoclonal Antibody (Catalog # IC63301A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
View Larger
Detection of GATA‑3 in MCF-7 cells by Flow Cytometry. MCF-7 cells were stained with Mouse Anti-Human GATA‑3 APC‑conjugated Monoclonal Antibody (Catalog # IC63301A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with FC012 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GATA-3
GATA-3 is a 54-60 kDa member of the GATA family of transcription regulating factors. There are currently six mammalian members, each of which binds to a G-A-T-A motif found in gene promoters. Although GATA-3 is traditionally described as being a hematopoietic transcriptional regulator, it has been found in multiple cell types, both embryonic and adult. Postnatal cells reported to express GATA-3 include NK cells, ILCs, NKT cells, B cells, thymocytes (DN, CD4 SP, and CD8 SP) and T cells, plus keratinocytes, sympathetic neurons, renal distal convoluted tubule and mammary duct epithelium. Human GATA-3 is 443 amino acids (aa) in length. It contains two GAGA-type Zn finger domains (aa 263-287 and 317-341) and multiple phosphorylation sites. GATAs as a group are known to either activate, or repress, gene expression, maintain transcriptional activity, and regulate gene expression levels. Within this framework, GATA-3 is best known to drive naïve CD4+ T cells into a Th2 phenotype, induce Th2 proliferation, and inhibit Th1 cell development via T-bet repression. Other effects attributed to GATA-33 include the promotion of Th9 and Treg formation, and the inhibition of Th1, TH17 and B cell development. Over aa 135-258, human and mouse GATA-3 share 94% aa sequence identity.
Product Datasheets
Citation for Human GATA-3 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Histone demethylase JARID1C/KDM5C regulates Th17 cells by increasing IL-6 expression in diabetic plasmacytoid dendritic cells
Authors: Audu, CO;Wolf, SJ;Joshi, AD;Moon, JY;Melvin, WJ;Sharma, SB;Davis, FM;Obi, AT;Wasikowski, R;Tsoi, LC;Barrett, EC;Mangum, KD;Bauer, TM;Kunkel, SL;Moore, BB;Gallagher, KA;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human GATA-3 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human GATA-3 APC-conjugated Antibody and earn rewards!
Have you used Human GATA-3 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
