Human GATA-3 Fluorescein-conjugated Antibody

Name: Human GATA-3 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: IC26051F

Catalog #: IC26051F Datasheet / COA / SDS

Discontinued Product

IC26051F has been discontinued.

View all GATA-3 products.

Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human GATA-3 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with rhGATA-1, -2, -4, -5, or -6.

Source

Monoclonal Mouse IgG₁ Clone # 291106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of GATA-3 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

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Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human GATA-3 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC26051F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Alternate Names

131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3