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Human GATA-5 Biotinylated Antibody

Catalog #: BAF2170 Datasheet / COA / SDS
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BAF2170
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Human GATA-5 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-5 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-2, rhGATA-3, rhGATA-4 and rhGATA-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GATA-5
Met1-Ala397
Accession # Q9BWX5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GATA‑5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-5

GATA-5 belongs to the GATA family of transcription factors which bind to the consensus DNA sequence (AIT) GATA (AIG) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA5 is important in the development and differentiation of endoderm-derived organs, including heart and gastrointestinal tissues. GATA-5 has also been implicated in cancer development (1, 2).

References
  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.
Entrez Gene IDs
140628 (Human)
Alternate Names
bB379O24.1; GATA binding factor-5; GATA binding protein 5; GATA5; GATA-5; GATA-binding factor 5; GATA-binding protein 5; transcription factor GATA-5

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