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Human GBX2 Antibody

Catalog #: AF4638
2 Citations Datasheet / COA / SDS

Discontinued Product

AF4638 has been discontinued.
View all GBX2 products.
Detection of Human GBX2 by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GBX2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GBX2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GBX2
Lys126-Asn247
Accession # P52951
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GBX2 antibody by Western Blot. View Larger

Detection of Human GBX2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human GBX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GBX2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GBX2

GBX2 (gastrulation brain homeobox) is a member of the homeobox family, which shares a 180 bp DNA sequence that encodes the DNA binding homeodomain. GBX2, along with OTX2 and FGF8 establish midbrain-hindbrain boundries in the developing brain. Increased GBX2 expression has also been associated with pluripotent cells and metastasic potential in cancers.

Long Name
Gastrulation Brain Homeobox 2
Entrez Gene IDs
2637 (Human); 14472 (Mouse); 114500 (Rat)
Alternate Names
Gastrulation and brain-specific homeobox protein 2; gastrulation brain homeo box 2; gastrulation brain homeobox 2; GBX2; homeobox protein GBX-2

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Citations for Human GBX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures
    Authors: JC Cossec, T Traboulsi, S Sart, Y Loe-Mie, M Guthmann, IA Hendriks, I Theurillat, ML Nielsen, ME Torres-Pad, CN Baroud, A Dejean
    Cell Reports, 2023-04-15;42(4):112380.
  2. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC

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