Human GDF-1 Antibody Summary
Asp254-Arg372
Accession # NP_001483
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human GDF‑1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GDF-1 Monoclonal Antibody (Catalog # MAB6937) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GDF-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-1
Growth and Differentiation Factor 1 (GDF‑1) is a secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that produces an approximately 44 kDa proprotein after cleavage of a 29 amino acid (aa) signal peptide. Further proteolysis at a dibasic cleavage site produces a 224 aa propeptide and a secreted 119 aa, 12‑15 kDa mature peptide (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7‑9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).
- Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
- Wall, N.A. et al. (2000) Dev. Biol. 227:495.
- Andersson, O. et al. (2007) Dev. Biol. 311:500.
- Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
- Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
- Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
- Andersson, O. et al. (2006) Dev. Biol. 293:370.
- Tanaka, C. et al. (2007) Genes Dev. 21:3272.
- Cheng, S.K. et al. (2003) Genes Dev. 17:31.
- Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.
Product Datasheets
Citation for Human GDF-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma
Authors: W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu
Nature Communications, 2021-12-08;12(1):7142.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human GDF-1 Antibody
There are currently no reviews for this product. Be the first to review Human GDF-1 Antibody and earn rewards!
Have you used Human GDF-1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
